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Microbiol. 16:1549030. doi: 10.3389/fmicb.2025.1549030 Author: Marc Rubio Creator: Adobe InDesign 14.0 (Windows) Producer: Adobe PDF Library 15.0 CreationDate: Thu Mar 6 06:29:40 2025 CET ModDate: Thu Mar 6 06:29:47 2025 CET Custom Metadata: no Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1852904 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- WKAYMB+MuseoSans-500 Type 1C Custom yes yes yes 765 0 UHWMKF+MuseoSans-300 Type 1C Custom yes yes yes 772 0 SEXAIJ+MuseoSans-700 Type 1C Custom yes yes yes 777 0 QBYOGN+MuseoSans-300Italic Type 1C WinAnsi yes yes yes 778 0 PXJISP+MinionPro-It Type 1C Custom yes yes yes 138 0 MVAQCV+MinionPro-Regular Type 1C Custom yes yes yes 131 0 JOMYMB+MuseoSans-500Italic Type 1C WinAnsi yes yes yes 105 0 IKXSYD+STIXGeneral-Regular Type 1C Custom yes yes yes 132 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-11-27 04:05:30 CET RepresentationInformation: 321774.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-11-26 13:01:45 CET Size: 1852904 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 821 FreeObjects: 356 IncrementalUpdates: 1 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-GB Outlines: Item: Title: Non-tuberculous mycobacteria isolates from patients with chronic pulmonary disease and no epidemiological relationship show sequence clusters through whole-genome sequencing Children: Item: Title: 1 Introduction Item: Title: 2 Methodology Children: Item: Title: 2.1 Patients and sample collection Item: Title: 2.2 Whole-genome sequencing Item: Title: 2.3 Bioinformatics pipeline Item: Title: 2.4 De novo assembly and species identification Item: Title: 2.5 Genome annotation and SGM distribution Item: Title: 2.6 Variant calling and phylogenetic analysis Item: Title: 2.7 Sequence cluster analysis Item: Title: 3 Results Children: Item: Title: 3.1 Slow-growing mycobacteria species identification Item: Title: 3.2 Sequence cluster analysis Item: Title: 4 Discussion Item: Title: References Info: Title: Non-tuberculous mycobacteria isolates from patients with chronic pulmonary disease and no epidemiological relationship show sequence clusters through whole-genome sequencing Author: Marc Rubio Subject: Rubio M, ​Fernandez-Pittol M, Batista S, Martínez D, San Nicolas L, ​Portell-Buj E, Busquets MA, Estelrich J, ​Gonzalez-Martin J, Navarro F, Tudó G and Garrigó M (2025) 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2025-03-06T10:59:40+05:30 2025-03-06T10:59:40+05:30 2025-03-06T10:59:47+05:30 2025-03-06T10:59:47+05:30 Adobe InDesign 14.0 (Windows) application/pdf Non-tuberculous mycobacteria isolates from patients with chronic pulmonary disease and no epidemiological relationship show sequence clusters through whole-genome sequencing Marc Rubio Mariana Fernandez-Pittol Sara Batista Diego Martínez Lorena San Nicolas Elena Portell-Buj Maria Antònia Busquets Joan Estelrich Julian Gonzalez-Martin Ferran Navarro Griselda Tudó Montserrat Garrigó Rubio M, ​Fernandez-Pittol M, Batista S, Martínez D, San Nicolas L, ​Portell-Buj E, Busquets MA, Estelrich J, ​Gonzalez-Martin J, Navarro F, Tudó G and Garrigó M (2025) Non-tuberculous mycobacteria isolates from patients with chronic pulmonary disease and no epidemiological relationship show sequence clusters through whole-genome sequencing. Front. Microbiol. 16:1549030. doi: 10.3389/fmicb.2025.1549030 non tuberculous mycobacteria whole genome sequencing molecular epidemiology cluster analysis bioinformatics Copyright © 2025 Rubio, Fernandez-Pittol, Batista, Martínez, San Nicolas, Portell-Buj, Busquets, Estelrich, Gonzalez-Martin, Navarro, Tudó and Garrigó. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 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