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Fecal intestinal inflammatory biomarkers in piglets: analytical validation, homogeneity and stability studies Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Experimental design and fecal sampling procedure Destination: mk:H2_1 Item: Title: 2.2 Fecal extraction procedure Destination: mk:H2_2 Item: Title: 2.3 Biomarker assays Destination: mk:H2_3 Item: Title: 2.4 Method validation Destination: mk:H2_4 Children: Item: Title: 2.4.1 Precision Destination: mk:H3_5 Item: Title: 2.4.2 Linearity under dilution Destination: mk:H3_6 Item: Title: 2.4.3 Recovery Destination: mk:H3_7 Item: Title: 2.4.4 Detection limits Destination: mk:H3_8 Item: Title: 2.5 Myeloperoxidase inhibition assay Destination: mk:H2_9 Item: Title: 2.6 Homogeneity of the biomarker distribution in fecal samples Destination: mk:H2_10 Item: Title: 2.7 Stability of the biomarkers in fecal samples and processed extracts under different storage conditions Destination: mk:H2_11 Item: Title: 2.8 Statistical analysis Destination: mk:H2_12 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 Assessment of precision Destination: mk:H2_13 Item: Title: 3.2 Assessment of linearity Destination: mk:H2_14 Item: Title: 3.3 Recovery study Destination: mk:H2_15 Item: Title: 3.4 Detection limits study Destination: mk:H2_16 Item: Title: 3.5 Myeloperoxidase inhibition assay Destination: mk:H2_17 Item: Title: 3.6 Homogeneity study Destination: mk:H2_18 Item: Title: 3.7 Stability study at different storage conditions Destination: mk:H2_19 Item: Title: 4 Discussion Destination: mk:H1_4 Children: Item: Title: 4.1 Analytical validation of fecal biomarkers Destination: mk:H2_20 Item: Title: 4.2 Homogeneity of the biomarker concentration in fecal samples Destination: mk:H2_21 Item: Title: 4.3 Stability of biomarkers at different storage conditions Destination: mk:H2_22 Item: Title: 4.4 Limitations of the study Destination: mk:H2_23 Item: Title: 5 Conclusions Destination: mk:H1_5 Item: Title: Funding Destination: mk:H1_6 Item: Title: Declaration of generative AI and AI-assisted technologies in the writing process Destination: mk:H1_7 Item: Title: Ethics statement Destination: mk:H1_8 Item: Title: Supplementary Figures Destination: mk:H1_9 Item: Title: CRediT authorship contribution statement Destination: mk:H1_10 Item: Title: Declaration of competing interest Destination: mk:HH1_2 Item: Title: Supplementary materials Destination: mk:H1_11 Item: Title: References Destination: mk:HH2_1 Info: Title: Fecal intestinal inflammatory biomarkers in piglets: analytical validation, homogeneity and stability studies Author: Anna Pelegrí-Pineda Subject: Veterinary and Animal Science, 30 (2025) 100523. doi:10.1016/j.vas.2025.100523 Keywords: Porcine,Biomarker,Feces,Non-invasive,Intestinal inflammation,Sample homogeneity Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) 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Veterinary and Animal Science, 30 (2025) 100523. doi:10.1016/j.vas.2025.100523 Porcine Biomarker Feces Non-invasive Intestinal inflammation Sample homogeneity Fecal intestinal inflammatory biomarkers in piglets: analytical validation, homogeneity and stability studies Anna Pelegrí-Pineda Raquel Pato Raquel Peña Júlia Suppi Eudald Llauradó-Calero David Solà-Oriol Anna Bassols Yolanda Saco VoR 22nd October 2025 Acrobat Distiller 8.1.0 (Windows) Porcine,Biomarker,Feces,Non-invasive,Intestinal inflammation,Sample homogeneity 22nd October 2025 10.1016/j.vas.2025.100523 noindex journal © 2025 The Authors. 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