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Title:           Intranasal inoculation with African swine fever virus genotype II reveals dose-dependent infectivity
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      Title: Intranasal inoculation with African swine fever virus genotype II reveals dose-dependent infectivity, pathogenesis and diagnostic challenges in pigs
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        Title: ABSTRACT
       Item: 
        Title: 1. Introduction
       Item: 
        Title: 2. Material and methods
        Children: 
         Item: 
          Title: 2.1. Cells and virus
         Item: 
          Title: 2.2. Experimental design
         Item: 
          Title: 2.3. ASFV detection by qPCR
         Item: 
          Title: 2.4. Thermal stability of bone marrow samples
         Item: 
          Title: 2.5. Detection of ASFV DNA by colorimetric LAMP in samples from group A
         Item: 
          Title: 2.6. Serum anti-ASFV antibodies detection
         Item: 
          Title: 2.7. Serum cytokines detection by Luminex assay
       Item: 
        Title: 3. Results
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         Item: 
          Title: 3.1. Clinical manifestations after inoculation with different ASFV doses
         Item: 
          Title: 3.2. ASFV DNA detection in clinical samples collected from pigs infected with different viral doses
         Item: 
          Title: 3.3. ASFV DNA detection in tissues
         Item: 
          Title: 3.4. ASFV DNA detection capacity in non-invasive samples
         Item: 
          Title: 3.5. ASFV detection in samples collected in the pen environment
         Item: 
          Title: 3.6. Thermal stability of ASFV in bone marrow of infected pigs
         Item: 
          Title: 3.7. ASFV DNA detection by colorimetric LAMP and qPCR in group a
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          Title: 3.8. ASFV-antibody response and cytokine profile in the three experimental groups
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        Title: 4. Discussion
       Item: 
        Title: Acknowledgments
       Item: 
        Title: Author contributions
       Item: 
        Title: Disclosure statement
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        Title: Funding
       Item: 
        Title: References
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       Rect: 193, 369, 325, 358
      Annotation: 
       Subtype: Link
       Rect: 130, 358, 149, 347
       ActionDest: 15
      Annotation: 
       Subtype: Link
       Rect: 82, 336, 217, 325
      Annotation: 
       Subtype: Link
       Rect: 126, 325, 144, 314
       ActionDest: 26
      Annotation: 
       Subtype: Link
       Rect: 153, 314, 310, 303
      Annotation: 
       Subtype: Link
       Rect: 226, 303, 245, 292
       ActionDest: 13
      Annotation: 
       Subtype: Link
       Rect: 145, 281, 163, 270
       ActionDest: 26
      Annotation: 
       Subtype: Link
       Rect: 344, 270, 506, 259
      Annotation: 
       Subtype: Link
       Rect: 130, 259, 148, 248
       ActionDest: 24
      Annotation: 
       Subtype: Link
       Rect: 121, 248, 253, 237
      Annotation: 
       Subtype: Link
       Rect: 122, 237, 141, 226
       ActionDest: 26
      Annotation: 
       Subtype: Link
       Rect: 306, 226, 475, 215
  Checksum: 498ca773
   Type: CRC32
  Checksum: bf2fd43883b16e55d33033828d33ea5b
   Type: MD5
  Checksum: 88f47e9e30fb54802ed9e58e7b67ae8827563aba
   Type: SHA-1
  Checksum: 1654df1da84a974477a03fcadba226fbf061544c397084326f59eb1cf3b2ec76
   Type: SHA-256