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UseOutlines Outlines: Item: Title: Development and validation of a novel Simoa assay for NPTX2 in Alzheimer’s disease and Down syndrome Destination: chapter..1 Children: Item: Title: Abstract Destination: section*.2 Item: Title: 1 | BACKGROUND Destination: section..1.1 Item: Title: 2 | MATERIALS AND METHODS Destination: section..1.2 Children: Item: Title: 2.1 | Study design and population Destination: subsection..1.2.1 Item: Title: 2.2 | CSF and plasma analysis Destination: subsection..1.2.2 Item: Title: 2.3 | Small extracellular vesicles (sEV) analysis Destination: subsection..1.2.3 Children: Item: Title: 2.3.1 | Isolation of total sEV (TE) from serum and NDE isolation from TE Destination: subsubsection..1.2.3.1 Item: Title: 2.4 | Nanoparticle tracking analyses (NTA) Destination: subsection..1.2.4 Item: Title: 2.5 | Preparation of human brain extract for Western blot analysis and IP-MS Destination: subsection..1.2.5 Item: Title: 2.6 | NPTX2 Simoa Destination: subsection..1.2.6 Item: Title: 2.7 | NPTX2 Western blot and gel electrophoresis Destination: subsection..1.2.7 Item: Title: 2.8 | NPTX2 mass spectrometry Destination: subsection..1.2.8 Item: Title: 2.9 | Image acquisition and processing Destination: subsection..1.2.9 Item: Title: 2.10 | Data processing and statistical analysis Destination: subsection..1.2.10 Item: Title: 3 | RESULTS Destination: section..1.3 Children: Item: Title: 3.1 | Validation of the CSF NPTX2 Simoa assay Destination: subsection..1.3.1 Item: Title: 3.2 | Validation of the plasma NPTX2 Simoa Destination: subsection..1.3.2 Item: Title: 3.3 | Correlations between MS and SIMOA assays for NPTX2 Destination: subsection..1.3.3 Item: Title: 3.4 | Characterization and presence of NPTX2 in CSF, brain, and plasma Destination: subsection..1.3.4 Item: Title: 3.5 | CSF NPTX2 in the BioFINDER pilot cohort Destination: subsection..1.3.5 Item: Title: 3.6 | CSF NPTX2 in the DABNI cohort Destination: subsection..1.3.6 Item: Title: 3.7 | NPTX2, cognition, brain atrophy, Ab-PET, and tau-PET Destination: subsection..1.3.7 Item: Title: 3.8 | Plasma NPTX2 Destination: subsection..1.3.8 Item: Title: 3.9 | NPTX2 in NDE Destination: subsection..1.3.9 Item: Title: 4 | DISCUSSION Destination: section..1.4 Item: Title: ACKNOWLEDGMENTS Destination: section*.4 Item: Title: CONFLICT OF INTEREST STATEMENT Destination: section*.6 Item: Title: CONSENT STATEMENT Destination: section*.8 Item: Title: REFERENCES Destination: section*.10 Item: Title: SUPPORTING INFORMATION Destination: section*.12 Info: Title: Development and validation of a novel Simoa assay for NPTX2 in Alzheimer's disease and Down syndrome Author: Subject: Keywords: Creator: LaTeX with hyperref package Producer: Acrobat Distiller 10.0.0 (Windows) CreationDate: Fri Jun 13 07:14:00 CEST 2025 ModDate: Fri Jun 13 07:14:59 CEST 2025 ID: 0x7db00f2f22a1225b5c40ac2be8a265ea, 0x2db0806a28ba48073682e893a098031c Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: 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