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Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Physicochemical characterization and preparation of PTFE-MNPLs dispersions Destination: mk:H2_1 Item: Title: 2.2 Intestinal cell models and culture conditions Destination: mk:H2_2 Item: Title: 2.3 Cell viability studies Destination: mk:H2_3 Item: Title: 2.4 Cell internalization by TEM and confocal imaging Destination: mk:H2_4 Item: Title: 2.5 Barrier integrity by TEER measurements Destination: mk:H2_5 Item: Title: 2.6 Inflammatory response Destination: mk:H2_6 Item: Title: 2.7 Mitochondrial membrane potential assay Destination: mk:H2_7 Item: Title: 2.8 Production of intracellular reactive oxygen species Destination: mk:H2_8 Item: Title: 2.9 Genotoxic and oxidative DNA damage by the comet assay Destination: mk:H2_9 Item: Title: 2.10 Statistical analysis Destination: mk:H2_10 Item: Title: 3 Results and discussion Destination: mk:H1_3 Children: Item: Title: 3.1 Physicochemical characterization of PTFE-MNPLs Destination: mk:H2_11 Item: Title: 3.2 Cell viability Destination: mk:H2_12 Item: Title: 3.3 Cellular internalization Destination: mk:H2_13 Item: Title: 3.4 Effect of PTFE-MNPLs on the intestinal barrier integrity Destination: mk:H2_14 Item: Title: 3.5 Pro-inflammatory effects of PTFE-MNPLs on intestinal epithelial models Destination: mk:H2_15 Item: Title: 3.6 Intracellular ROS production and mitochondrial membrane potential Destination: mk:H2_16 Item: Title: 3.7 Genotoxic and oxidative DNA damage Destination: mk:H2_17 Item: Title: 4 Conclusion Destination: mk:H1_4 Item: Title: Environmental implication Destination: mk:H1_5 Item: Title: Funding Destination: mk:H1_6 Item: Title: CRediT authorship contribution statement Destination: mk:H1_7 Item: Title: Declaration of Competing Interest Destination: mk:HH1_3 Item: Title: Acknowledgment Destination: mk:HH1_4 Item: Title: Appendix A Supporting 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Journal of Hazardous Materials, 499 (2025) 140255. doi:10.1016/j.jhazmat.2025.140255 Teflon™ Micro/nanoplastics Human Intestinal barrier Oxidative stress Genotoxicity Mitochondrial damage Polytetrafluoroethylene (PTFE, Teflon) microplastics and nanoplastics induce oxidative stress, mitochondrial damage, and genotoxicity in human intestinal cells Doaa Abass Mohamed Alaraby Elham Farghal Elkady Michelle Morataya-Reyes Gooya Banaei Joan Martín-Pérez Laura Rubio Irene Barguilla Ricard Marcos Alba Hernández Alba García-Rodríguez VoR 18th November 2025 Acrobat Distiller 8.1.0 (Windows) Teflon™,Micro/nanoplastics,Human,Intestinal barrier,Oxidative stress,Genotoxicity,Mitochondrial damage 18th November 2025 sciencedirect.com elsevier.com true 2010-04-23 10.1016/j.jhazmat.2025.140255 noindex journal 2025-11-05 5 November 2025 10.1016/j.jhazmat.2025.140255 0304-3894 140255 Journal of Hazardous Materials 140255 https://doi.org/10.1016/j.jhazmat.2025.140255 499 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