029924d3b43789f4bf67225b19de5d10 ijms-26-11450-v3.pdf 4893b67405594b8d8369ba1a5906cda37f4c9c60 ijms-26-11450-v3.pdf b8878bbec9c61000ba8047ed9c28d9f256c84d86a3564d77d6dd305d7e93bc8d ijms-26-11450-v3.pdf Title: Transcriptional Responses to Alkaline pH Across Fungi: Common and Differential Features, and Biotechnological Applications Subject: The transcriptional response to alkalinization in Saccharomyces cerevisiae, Aspergillus nidulans and Candida albicans raised the interest of the scientific community many years ago for diverse reasons, and the underlying signaling pathways have been elucidated in these organisms in detail. Within the last few years, transcriptomic data for other fungal species have become available, although in most cases little is known about the molecular basis controlling their adaptive response. The objective of this work is to provide an overview on how different fungi remodel their gene expression in response to environmental alkalinization, highlighting the similitudes and differences among them. Microbial stress-responsive promoters have been considered useful tools for biotechnological applications, such as expression of recombinant proteins of industrial interest. Recent work, emphasizing the usefulness of alkaline pH-inducible promoters for heterologous protein production, will also be discussed. Keywords: transcriptional responses; environmental alkalinization; gene regulation; fungi; biotechnological applications Author: Joaquín Ariño Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25; modified using OpenPDF 1.4.2 CreationDate: Mon Dec 1 05:09:27 2025 CET ModDate: Mon Dec 1 05:15:09 2025 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 25 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1082156 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XFRWTU+URWPalladioL-Ital Type 1 Custom yes yes yes 117 0 QKZKVH+VnURWPalladioL Type 1 Custom yes yes yes 118 0 TQPKHE+URWPalladioL-Bold Type 1 Custom yes yes yes 119 0 YZXBBX+URWPalladioL-Roma Type 1 Custom yes yes yes 120 0 EILUJF+CMSY10 Type 1 Builtin yes yes yes 204 0 MPUDFL+PazoMath Type 1 Builtin yes yes yes 233 0 GVKTTX+URWPalladioL-BoldItal Type 1 Custom yes yes yes 320 0 CPGPBG+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 394 0 CPGPBE+PalatinoLinotype TrueType WinAnsi yes yes no 395 0 CPGPBE+PalatinoLinotype TrueType WinAnsi yes yes no 474 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-01-23 03:08:24 CET RepresentationInformation: ijms-26-11450-v3.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-01-22 11:25:42 CET Size: 1082156 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 657 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: The Transcriptional Response to High pH Across Fungi Children: Item: Title: Saccharomyces cerevisiae Item: Title: Aspergillus nidulans Item: Title: Aspergillus fumigatus Item: Title: Aspergillus niger Item: Title: Candida albicans Item: Title: Schizosaccharomyces pombe Item: Title: Debaryomyces hansenii Item: Title: Aureobasidium pullulans Item: Title: Trichoderma sp. Item: Title: Ustilago maydis Item: Title: Komagataella phaffii Item: Title: An Integrative Overview of the Transcriptional Responses to Alkaline pH Item: Title: Linking the Alkaline Transcriptional Response to Biotechnological Applications Item: Title: Conclusions Item: Title: References Info: Title: Transcriptional Responses to Alkaline pH Across Fungi: Common and Differential Features, and Biotechnological Applications Author: Joaquín Ariño Subject: The transcriptional response to alkalinization in Saccharomyces cerevisiae, Aspergillus nidulans and Candida albicans raised the interest of the scientific community many years ago for diverse reasons, and the underlying signaling pathways have been elucidated in these organisms in detail. Within the last few years, transcriptomic data for other fungal species have become available, although in most cases little is known about the molecular basis controlling their adaptive response. The objective of this work is to provide an overview on how different fungi remodel their gene expression in response to environmental alkalinization, highlighting the similitudes and differences among them. Microbial stress-responsive promoters have been considered useful tools for biotechnological applications, such as expression of recombinant proteins of industrial interest. Recent work, emphasizing the usefulness of alkaline pH-inducible promoters for heterologous protein production, will also be discussed. 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