63d18fa50f0589c6104bba3475dc8c4e antioxidants-14-01489.pdf 3abfb735e8fa436522a160f3199604fe2f643b3b antioxidants-14-01489.pdf f277df5f72f3cbb4ce1609be9d79f365bdee67c088d5e3f04596337a21aaced7 antioxidants-14-01489.pdf Title: Supplementation with Polyphenols (Olive and Grape Extracts) Improves Pregnancy Outcomes in Underfed Sheep Subject: Our study aimed to test the hypothesis that supplementation with a combination of dry grape extracts (DGS) and olive-originated hydroxityrosol (HxT) polyphenols during ovine gestation under moderate nutritional restriction allows for the improvement of the birth weight and size of newborn lambs, especially in more demanding pregnancies such as twins. Twenty-six pregnant ewes (fourteen ewes with singleton pregnancies and twelve ewes with twin pregnancies) were divided in two equal groups, treated or not with the polyphenols, and involved in the study. The results indicate that supplementation with the combination of polyphenols, without modifying maternal body weight and body condition score, increased the maternal total antioxidant capacity (p < 0.05) and, interacting with pregnancy rank, improved maternal metabolic status by reducing -hydroxybutyrate and non-esterified fatty acids (p < 0.05). Afterwards, supplementation improved birth weight and size of newborns in the most compromised pregnancies (i.e., twin pregnancies; twins lambs born to supplemented ewes were significantly heavier than those born to control ewes (3.3 0.13 vs. 2.8 0.23 kg, respectively; p < 0.05). Keywords: antioxidants; undernutrition; sheep gestation; birth weight Author: José Luis Pesántez, Paula Martínez-Ros, Antonio González-Bulnes, M. Carmen López-Mendoza, Francisco Sales, Nesrein M. Hashem, Mónica De los Reyes, Luis A. Raggi, Natalia Yeste-Vizcaíno and Víctor H. Parraguez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25; modified using OpenPDF 1.4.2 CreationDate: Fri Dec 12 03:43:21 2025 CET ModDate: Fri Dec 12 03:46:36 2025 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 669591 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EBYWGN+URWPalladioL-Bold Type 1 Custom yes yes yes 140 0 FDUXRJ+URWPalladioL-Roma Type 1 Custom yes yes yes 141 0 YYCRJS+URWPalladioL-Ital Type 1 Custom yes yes yes 142 0 CAMXTK+EURM10 Type 1 Builtin yes yes yes 143 0 XEYJZU+CMSY10 Type 1 Builtin yes yes yes 144 0 OJKEAJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 221 0 OJKEAH+PalatinoLinotype TrueType WinAnsi yes yes no 222 0 OJKEAF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 223 0 OJKEAJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 232 0 OJKEAH+PalatinoLinotype TrueType WinAnsi yes yes no 233 0 OJKEAF+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 234 0 OJKELG+Symbol Type 1C Custom yes yes yes 235 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 269 0 OJKEAJ+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 263 0 OJKGCC+TimesNewRoman,Bold TrueType WinAnsi yes yes no 264 0 OJKEAH+PalatinoLinotype TrueType WinAnsi yes yes no 265 0 OJKGCE+TimesNewRoman TrueType WinAnsi yes yes no 266 0 QKSNNP+VnURWPalladioL Type 1 Custom yes yes yes 424 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-01-23 03:08:20 CET RepresentationInformation: antioxidants-14-01489.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-01-22 11:47:28 CET Size: 669591 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 493 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Ethics, Animals, and General Management Item: Title: Analysis of Blood and Colostrum Samples Item: Title: Statistical Analysis Item: Title: Results Children: Item: Title: Maternal Traits Children: Item: Title: Changes in Maternal BW and BCS over Time of Pregnancy Item: Title: Assessment of Maternal Antioxidant/Oxidative Status at 140 Days of Pregnancy Item: Title: Assessment of Maternal Metabolic Biomarkers Item: Title: Assessment of Colostrum Composition Item: Title: Newborn Traits Children: Item: Title: Birth Weight Item: Title: Body Size at Birth Item: Title: Discussion Children: Item: Title: Changes in Maternal Body Weight, Body Condition, and Metabolic Status Item: Title: Changes in Newborn Lambs’ Body Weight and Size Item: Title: Changes in Colostrum Item: Title: Conclusions Item: Title: References Info: Title: Supplementation with Polyphenols (Olive and Grape Extracts) Improves Pregnancy Outcomes in Underfed Sheep Author: José Luis Pesántez, Paula Martínez-Ros, Antonio González-Bulnes, M. Carmen López-Mendoza, Francisco Sales, Nesrein M. Hashem, Mónica De los Reyes, Luis A. Raggi, Natalia Yeste-Vizcaíno and Víctor H. Parraguez Subject: Our study aimed to test the hypothesis that supplementation with a combination of dry grape extracts (DGS) and olive-originated hydroxityrosol (HxT) polyphenols during ovine gestation under moderate nutritional restriction allows for the improvement of the birth weight and size of newborn lambs, especially in more demanding pregnancies such as twins. Twenty-six pregnant ewes (fourteen ewes with singleton pregnancies and twelve ewes with twin pregnancies) were divided in two equal groups, treated or not with the polyphenols, and involved in the study. The results indicate that supplementation with the combination of polyphenols, without modifying maternal body weight and body condition score, increased the maternal total antioxidant capacity (p < 0.05) and, interacting with pregnancy rank, improved maternal metabolic status by reducing -hydroxybutyrate and non-esterified fatty acids (p < 0.05). Afterwards, supplementation improved birth weight and size of newborns in the most compromised pregnancies (i.e., twin pregnancies; twins lambs born to supplemented ewes were significantly heavier than those born to control ewes (3.3 0.13 vs. 2.8 0.23 kg, respectively; p < 0.05). 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