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Endocrinol. 16:1706886. doi: 10.3389/fendo.2025.1706886 Keywords: incident type 2 diabetes; liquid chromatography; mass spectrometry; untargeted metabolomics; targeted metabolomics Author: Maria Barranco-Altirriba CreationDate: Tue Dec 2 02:10:29 2025 CET ModDate: Tue Dec 2 02:10:29 2025 CET Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.389 x 841.663 pts (A4) Page rot: 0 File size: 3438968 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BDMJNA+AdvTTd3a5f740 Type 1C WinAnsi yes yes no 458 0 BDMKND+AdvTTd168d80a.I Type 1C WinAnsi yes yes no 700 0 BDMJNB+AdvPSA18C Type 1C WinAnsi yes yes no 701 0 BDMJNC+AdvTTd3a5f740+01 Type 1C MacRoman yes yes no 702 0 BDMJND+AdvTT9b12cd41 Type 1C WinAnsi yes yes no 459 0 BDMJNE+AdvPSA323 Type 1C WinAnsi yes yes no 703 0 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bmark_1934186206 Children: Item: Title: Highlights Destination: s1 Item: Title: Introduction Destination: s2 Item: Title: Methods Destination: s3 Children: Item: Title: Study populations Destination: s3_1 Item: Title: Discovery phase cohort Destination: s3_2 Item: Title: Validation phase cohort Destination: s3_3 Item: Title: Untargeted analysis Destination: s3_4 Children: Item: Title: Metabolite extraction Destination: s3_4_1 Item: Title: LC-MS settings Destination: s3_4_2 Item: Title: Metabolite identification by MS/MS Destination: s3_4_3 Item: Title: Data processing Destination: s3_4_4 Item: Title: Targeted analysis Destination: s3_5 Children: Item: Title: Metabolite extraction Destination: s3_5_1 Item: Title: LC-MS settings Destination: s3_5_2 Item: Title: Targeted method settings Destination: s3_5_3 Item: Title: Statistical analysis—discovery Destination: s3_6 Item: Title: Statistical analysis—validation Destination: s3_7 Item: Title: Results Destination: s4 Children: Item: Title: Data preparation Destination: s4_1 Item: Title: Baseline characteristics Destination: s4_2 Item: Title: Analysis in the discovery phase Destination: s4_3 Item: Title: Validation phase Destination: s4_4 Item: Title: Discussion Destination: s5 Item: Title: Data availability statement Destination: bmark_-1258588262 Item: Title: Ethics statement Destination: bmark_1370551237 Item: Title: Author contributions Destination: bmark_-1208584890 Item: Title: Funding Destination: bmark_1865464027 Item: Title: Acknowledgments Destination: bmark_-366698314 Item: Title: Conflict of interest Destination: bmark_1089982005 Item: Title: Generative AI statement Destination: bmark_-1453097031 Item: Title: Publisher’s note Destination: bmark_-1256977648 Item: Title: Supplementary material Destination: bmark_632582120 Item: Title: References Destination: bmark_2090415954 Info: Title: Guanine and pregnenolone sulfate are associated with incident type 2 diabetes in two independent populations Author: Maria Barranco-Altirriba Subject: Barranco-Altirriba M, Granado-Casas M, Yanes O, Capellades J, Junza A, Franch-Nadal J, Vendrell J, Llauradó G, Valdés S, García-Escobar E, Bermúdez-López M, Valdivielso JM, López-Lifante V-M, Herrero-Alonso C, Falguera M, Vilanova MB, Arteaga I, Torán-Monserrat P, Perera-Lluna A, Castelblanco E and Mauricio D (2025) Guanine and pregnenolone sulfate are associated with incident type 2 diabetes in two independent populations. Front. 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incident type 2 diabetes in two independent populations. Front. Endocrinol. 16:1706886. doi: 10.3389/fendo.2025.1706886 Copyright © 2025 Barranco-Altirriba, Granado-Casas, Yanes, Capellades, Junza, Franch-Nadal, Vendrell, Llauradó, Valdés, García-Escobar, Bermúdez-López, Valdivielso, López-Lifante, Herrero-Alonso, Falguera, Vilanova, Arteaga, Torán-Monserrat, Perera-Lluna, Castelblanco and Mauricio.. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. incident type 2 diabetes liquid chromatography mass spectrometry untargeted metabolomics targeted metabolomics Guanine and pregnenolone sulfate are associated with incident type 2 diabetes in two independent populations incident type 2 diabetes; liquid chromatography; mass spectrometry; untargeted metabolomics; targeted metabolomics True https://www.frontiersin.org/legal/copyright-statement 2025-12-02T09:10:29+08:00 2025-12-02T09:10:29+08:00 2025-12-02T09:10:29+08:00 uuid:fca29063-83ae-4fb5-9222-fc755fc000c8 uuid:98de43cb-0393-4c4e-9241-c40cbcec244d Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 221, 685, 486, 706 Annotation: Subtype: Link Rect: 221, 660, 478, 681 Annotation: Subtype: Link Rect: 221, 635, 520, 656 Annotation: Subtype: Link Rect: 221, 610, 463, 631 Annotation: Subtype: Link Rect: 49, 33, 135, 40 Annotation: Subtype: Link Rect: 500, 33, 545, 40 Annotation: 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