6e79f865dfa1a950302edda3e56433d3 327674.pdf de9753365e8de8af0cd62bd59285eb73067b5a25 327674.pdf f0c8dc2320f458feddeb18242672f8e5f37c1c7a7e8ba61be081cc855e836b4b 327674.pdf Title: Long-Term Biochemical and Cardiovascular Profiles 3–6 Years After Preeclampsia: Impact of Angiogenic Imbalance During Pregnancy Subject: Background/Objectives: Preeclampsia is associated with long-term cardiovascular and metabolic risks. This study aimed to evaluate metabolic and cardiovascular biochemical profiles in women with a history of preeclampsia and angiogenic imbalance during pregnancy. Methods: We conducted a cross-sectional study at Hospital de la Santa Creu i Sant Pau between August 2023 and July 2025. Participants had been prospectively enrolled during pregnancy (2018–2022) and were re-evaluated 3 to 6 years later. Blood and urine samples were collected after a 12-h fast to assess hematological, metabolic, and cardiovascular markers. Angiogenic profiles were determined using sFlt-1/PlGF ratios obtained during pregnancy. Multivariable linear regression models were used to assess associations with a history of PE and angiogenic imbalance, adjusting for relevant confounders. Results: 363 participants were included. 113 (31.1%) had a history of preeclampsia. Women with previous preeclampsia showed slightly higher high-sensitivity troponin T concentrations [4.0 (3.0–6.0) ng/L vs. 3.2 (3.0–5.0) ng/L, p = 0.03]. Women with sFlt-1/PlGF 38 exhibited significantly higher urinary protein [0.09 (0.07–0.18) g/L vs. 0.08 (0.07–0.13) g/L, p = 0.01], potassium [4.25 (4.07–4.40) mmol/L vs. 4.19 (4.02–4.37) mmol/L, p = 0.048], and LDH concentrations [168 (150–189) U/L vs. 163 (149–177) U/L, p = 0.046], and lower leukocyte counts [6150 (5348–7055) vs. 6250 (5430–7450) U/mL, p = 0.03]. Conclusions: Women with angiogenic imbalance during pregnancy display subtle alterations in renal and endothelial function markers years after delivery, whereas those with preeclampsia show slightly higher troponin concentrations. These findings, though clinically irrelevant, suggest that pregnancy-related vascular dysfunction may have different long-term manifestations depending on whether the maternal cardiovascular system was sufficiently compromised to develop overt preeclampsia. Keywords: preeclampsia; sFlt-1; PlGF; cardiovascular risk; endothelial dysfunction; metabolic profile; biomarkers Author: Noah Costa, Judit Platero, Pablo Garcia-Manau, Olga Sanchez-Garcia, Clàudia Pellicer, Mariona Jordi, Zoraida Garcia, Carmen Garrido-Gimenez, Johana Ullmo, Madalina Nan, Josefina Mora, Alvaro Garcia-Osuna, Marta Choliz, Monica Cruz-Lemini, Maria del Carmen Medina and Elisa Llurba Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25; modified using OpenPDF 1.4.2 CreationDate: Wed Nov 26 13:24:24 2025 CET ModDate: Wed Nov 26 14:47:32 2025 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 416335 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QRMLDG+URWPalladioL-Bold Type 1 Custom yes yes yes 87 0 WNWTFR+VnURWPalladioL-Bold Type 1 Custom yes yes yes 88 0 OHXQCJ+CMSY10 Type 1 Builtin yes yes yes 89 0 QKZKVH+VnURWPalladioL Type 1 Custom yes yes yes 90 0 DIJDQQ+URWPalladioL-Roma Type 1 Custom yes yes yes 91 0 YAKXKL+URWPalladioL-Ital Type 1 Custom yes yes yes 92 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes yes 173 0 FGOJKC+PalatinoLinotype TrueType WinAnsi yes yes no 198 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-05-06 04:09:42 CEST RepresentationInformation: 327674.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-05-05 13:31:10 CEST Size: 416335 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 345 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: Materials and Methods Item: Title: Results Item: Title: Discussion Children: Item: Title: Main Findings Item: Title: Comparison with Other Studies Item: Title: Clinical Implications Item: Title: Strengths and Limitations Item: Title: Conclusions Item: Title: References Info: Title: Long-Term Biochemical and Cardiovascular Profiles 3–6 Years After Preeclampsia: Impact of Angiogenic Imbalance During Pregnancy Author: Noah Costa, Judit Platero, Pablo Garcia-Manau, Olga Sanchez-Garcia, Clàudia Pellicer, Mariona Jordi, Zoraida Garcia, Carmen Garrido-Gimenez, Johana Ullmo, Madalina Nan, Josefina Mora, Alvaro Garcia-Osuna, Marta Choliz, Monica Cruz-Lemini, Maria del Carmen Medina and Elisa Llurba Subject: Background/Objectives: Preeclampsia is associated with long-term cardiovascular and metabolic risks. This study aimed to evaluate metabolic and cardiovascular biochemical profiles in women with a history of preeclampsia and angiogenic imbalance during pregnancy. Methods: We conducted a cross-sectional study at Hospital de la Santa Creu i Sant Pau between August 2023 and July 2025. Participants had been prospectively enrolled during pregnancy (2018–2022) and were re-evaluated 3 to 6 years later. Blood and urine samples were collected after a 12-h fast to assess hematological, metabolic, and cardiovascular markers. Angiogenic profiles were determined using sFlt-1/PlGF ratios obtained during pregnancy. Multivariable linear regression models were used to assess associations with a history of PE and angiogenic imbalance, adjusting for relevant confounders. Results: 363 participants were included. 113 (31.1%) had a history of preeclampsia. Women with previous preeclampsia showed slightly higher high-sensitivity troponin T concentrations [4.0 (3.0–6.0) ng/L vs. 3.2 (3.0–5.0) ng/L, p = 0.03]. Women with sFlt-1/PlGF 38 exhibited significantly higher urinary protein [0.09 (0.07–0.18) g/L vs. 0.08 (0.07–0.13) g/L, p = 0.01], potassium [4.25 (4.07–4.40) mmol/L vs. 4.19 (4.02–4.37) mmol/L, p = 0.048], and LDH concentrations [168 (150–189) U/L vs. 163 (149–177) U/L, p = 0.046], and lower leukocyte counts [6150 (5348–7055) vs. 6250 (5430–7450) U/mL, p = 0.03]. Conclusions: Women with angiogenic imbalance during pregnancy display subtle alterations in renal and endothelial function markers years after delivery, whereas those with preeclampsia show slightly higher troponin concentrations. These findings, though clinically irrelevant, suggest that pregnancy-related vascular dysfunction may have different long-term manifestations depending on whether the maternal cardiovascular system was sufficiently compromised to develop overt preeclampsia. 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