617b119bd39cf90ee33c926280db2883 diagnostics-15-02960.pdf 1071b2fdaaad2290b5cdd787f5fabe026790eaad diagnostics-15-02960.pdf ee9f374c93187e6c72c4441c57707e32af15959958a652f691dd4a30ded99644 diagnostics-15-02960.pdf Title: Characterization of Infarct Size and Remodeling Using CMR and PET in Mice Models of Reperfused and Non-Reperfused Myocardial Infarction Subject: Background/Objectives: Unlike post-mortem histopathology, cardiovascular magnetic resonance (CMR) and positron emission tomography (PET) enable longitudinal assessment of structural, functional, and metabolic alterations in preclinical myocardial infarction models. This study aims to describe the temporal evolution of infarct size and systolic function by CMR and glucose consumption via PET, explore their differences in non-reperfused and reperfusion infarction models, and assess their capacity to predict histology-derived infarct size and systolic function at chronic phase CMR. Methods: Two murine models of myocardial infarction were generated using permanent (non-reperfused, n = 8) or transient (reperfused, n = 9) coronary occlusion. CMR and fluorine-18 2-fluoro-2-deoxyglucose PET imaging were performed at baseline and at 1, 7, and 21 days post-infarction to quantify infarct size, systolic function, and myocardial glucose metabolism. Infarct size was also assessed using Masson’s trichrome staining. Results: At 24 h post-infarction, CMR-derived infarction together with significant reduction in systolic function and glucose metabolism were already noted in both models. At 21-day CMR, however, reperfused mice showed lower infarct size and more preserved systolic function compared to their non-reperfused counterparts, while no differences in glucose metabolism were reported. Infarct size and systolic function at 1-day CMR and the number of segments with reduced glucose consumption at 1-day PET independently predicted histology-derived infarct size and long-term systolic function. Conclusions: Combined PET/CMR imaging enables non-invasive, sequential evaluation of infarct size, systolic function, and glucose metabolism in experimental myocardial infarction. This multimodality approach is well suited for assessing the efficacy of emerging therapies in preclinical research. Keywords: magnetic resonance; myocardial infarction; positron emission tomography Author: Jose Gavara, Tamara Molina-Garcia, Mustafa Ezzeddin, Ana Diaz, Nerea Perez-Sole, Maria Ortega, Victor Marcos-Garces, Elena de Dios, Antoni Bayes-Genis, Amparo Ruiz-Sauri, Cesar Rios-Navarro and Vicente Bodi Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25; modified using OpenPDF 1.4.2 CreationDate: Sat Nov 22 05:23:01 2025 CET ModDate: Sat Nov 22 05:27:03 2025 CET Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4270611 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BMYDNX+URWPalladioL-Roma Type 1 Custom yes yes yes 129 0 PMACRG+URWPalladioL-Ital Type 1 Custom yes yes yes 130 0 STOLDD+URWPalladioL-Bold Type 1 Custom yes yes yes 131 0 UWJUYN+CMSY10 Type 1 Builtin yes yes yes 168 0 OVNEXW+EURM10 Type 1 Builtin yes yes yes 204 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes yes 212 0 HEHGCD+PalatinoLinotype TrueType WinAnsi yes yes no 224 0 ANMNFH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 269 0 ANMNFF+PalatinoLinotype TrueType WinAnsi yes yes no 270 0 ANMNFF+PalatinoLinotype TrueType WinAnsi yes yes no 280 0 SQEBUP+VnURWPalladioL Type 1 Custom yes yes yes 404 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-04-21 04:15:04 CEST RepresentationInformation: diagnostics-15-02960.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-04-20 15:55:22 CEST Size: 4270611 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 484 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Mouse MI Model Item: Title: CMR/18F-2-Fluoro-2-deoxyglucose PET Protocol Item: Title: CMR Acquisition and Quantification Item: Title: PET Acquisition and Quantification Item: Title: Histological Analysis Item: Title: Statistical Analysis Item: Title: Results Children: Item: Title: CMR Imaging Item: Title: 18F-2-Fluoro-2-deoxyglucose PET Imaging Item: Title: Prognostic Value of CMR/PET Indices for Histological Infarct Size Item: Title: Prognostic Value of 1-Day CMR/PET Indices for LV Remodeling Item: Title: Discussion Children: Item: Title: Importance of Non-Invasive Imaging Techniques in Experimental Models of MI Item: Title: Longitudinal CMR Changes and Comparison Between Permanent and Transient Coronary Ischemia Item: Title: Sequential PET Changes in Murine MI Models Item: Title: Study Limitations Item: Title: Conclusions Item: Title: References Info: Title: Characterization of Infarct Size and Remodeling Using CMR and PET in Mice Models of Reperfused and Non-Reperfused Myocardial Infarction Author: Jose Gavara, Tamara Molina-Garcia, Mustafa Ezzeddin, Ana Diaz, Nerea Perez-Sole, Maria Ortega, Victor Marcos-Garces, Elena de Dios, Antoni Bayes-Genis, Amparo Ruiz-Sauri, Cesar Rios-Navarro and Vicente Bodi Subject: Background/Objectives: Unlike post-mortem histopathology, cardiovascular magnetic resonance (CMR) and positron emission tomography (PET) enable longitudinal assessment of structural, functional, and metabolic alterations in preclinical myocardial infarction models. This study aims to describe the temporal evolution of infarct size and systolic function by CMR and glucose consumption via PET, explore their differences in non-reperfused and reperfusion infarction models, and assess their capacity to predict histology-derived infarct size and systolic function at chronic phase CMR. Methods: Two murine models of myocardial infarction were generated using permanent (non-reperfused, n = 8) or transient (reperfused, n = 9) coronary occlusion. CMR and fluorine-18 2-fluoro-2-deoxyglucose PET imaging were performed at baseline and at 1, 7, and 21 days post-infarction to quantify infarct size, systolic function, and myocardial glucose metabolism. Infarct size was also assessed using Masson’s trichrome staining. Results: At 24 h post-infarction, CMR-derived infarction together with significant reduction in systolic function and glucose metabolism were already noted in both models. At 21-day CMR, however, reperfused mice showed lower infarct size and more preserved systolic function compared to their non-reperfused counterparts, while no differences in glucose metabolism were reported. Infarct size and systolic function at 1-day CMR and the number of segments with reduced glucose consumption at 1-day PET independently predicted histology-derived infarct size and long-term systolic function. Conclusions: Combined PET/CMR imaging enables non-invasive, sequential evaluation of infarct size, systolic function, and glucose metabolism in experimental myocardial infarction. This multimodality approach is well suited for assessing the efficacy of emerging therapies in preclinical research. 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