6a909883cbd28f16aadb77402492eea5 328095.pdf debd7e18bb397377d308d23830fa2da2701265e9 328095.pdf 18513edc8efbda187491da7fa35010a9660ea285df40a185f7081b4b6b53a9a2 328095.pdf Title: Estimating the Contribution of Renal Function to Endothelial Dysfunction and Subclinical Inflammation with a Two-Cohort Study: Living Kidney Donors and Their Transplant Recipients Subject: Living kidney transplantation offers the best results for end-stage renal disease patients, but concerns about cardiovascular risk after nephrectomy for kidney donors have been raised. We aimed to estimate the contribution of renal function to endothelial dysfunction (ED) and subclinical inflammation in a non-interventional, prospective, multicenter, longitudinal study with two cohorts: living kidney donors and their transplant recipients (registered clinical trial NCT02515643). The measured glomerular filtration rate (mGFR) by iohexol clearance, estimated GFR according to the CKD-EPI and MDRD-4 formulas, and levels of endothelial dysfunction (sVCAM-1, sICAM-1, E-selectin, von Willebrand Factor, pentraxin, and urinary albumin-to-creatinine ratio) and subclinical inflammation biomarkers (sIL-6, sTNF-R1, sTNF-R2, sTWEAK, and high-sensitivity C-reactive protein) were determined at baseline and 1-year follow-up. Fifty pairs of donors and recipients were recruited between 2015 and 2018. Among the endothelial dysfunction biomarkers, sVCAM-1 increased in donors and decreased in recipients (p < 0.01) while, among the inflammation biomarkers, sTNFR1 and sTNFR2 significantly increased in donors and decreased in recipients (p < 0.001). After transplantation, parallel increases and decreases in ED and subclinical inflammation biomarkers were observed in the donor and recipient cohorts, respectively. Long-term follow-up is needed to characterize the cardiovascular risk associated with these changes. Keywords: kidney transplantation; living donor; renal function; iohexol clearance; endothelial dysfunction; inflammation Author: Irina B. Torres, Carla Burballa, José M. González-Posada, Domingo Hernández, Esteban Porrini, Janire Perurena, Vicente Cortina, Manel Perelló, Dolores Redondo-Pachón, Ana González-Rine, Mercedes Cabello, Maria José Pérez-Sáez, Marta Crespo, Oriol Bestard, Daniel Serón and Francesc Moreso Creator: LaTeX with hyperref Producer: pdfTeX-1.40.25; modified using OpenPDF 1.4.2 CreationDate: Mon Sep 29 14:58:07 2025 CEST ModDate: Mon Sep 29 15:01:44 2025 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 17 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1565076 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MIKRSI+VnURWPalladioL Type 1 Custom yes yes yes 120 0 IADWPK+URWPalladioL-Bold Type 1 Custom yes yes yes 121 0 UEWYZJ+URWPalladioL-Roma Type 1 Custom yes yes yes 122 0 NOVKLB+URWPalladioL-Ital Type 1 Custom yes yes yes 123 0 CEBMDP+PalatinoLinotype TrueType WinAnsi yes yes no 189 0 CEBMDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 190 0 GQWIGS+CMSY10 Type 1 Builtin yes yes yes 215 0 MYDOFE+EURM10 Type 1 Builtin yes yes yes 234 0 CEBMDP+PalatinoLinotype TrueType WinAnsi yes yes no 227 0 CEBMDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 228 0 CEBMDL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 229 0 CEBMDP+PalatinoLinotype TrueType WinAnsi yes yes no 256 0 CEBMDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 257 0 CEBMDL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 258 0 CEBMDP+PalatinoLinotype TrueType WinAnsi yes yes no 287 0 CEBMDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 288 0 CEBMDP+PalatinoLinotype TrueType WinAnsi yes yes no 296 0 CEBMDN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 297 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-05-05 04:08:21 CEST RepresentationInformation: 328095.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-05-04 12:29:21 CEST Size: 1565076 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 500 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Item: Title: Results Children: Item: Title: Patient Characteristics Item: Title: Renal Function at One Year Item: Title: Endothelial Dysfunction and Inflammation Biomarkers Item: Title: Relationship Between Renal Function and Biomarkers Item: Title: Blood Pressure and Atherosclerotic Burden in the Donor Cohort Item: Title: Follow-Up at 5 Years Item: Title: Discussion Item: Title: Materials and Methods Children: Item: Title: Study Design Item: Title: Patients Item: Title: Study Procedures Item: Title: Treatments Item: Title: Biomarkers of Endothelial Dysfunction and Chronic Inflammation Item: Title: Data Analysis Item: Title: Conclusions Item: Title: References Info: Title: Estimating the Contribution of Renal Function to Endothelial Dysfunction and Subclinical Inflammation with a Two-Cohort Study: Living Kidney Donors and Their Transplant Recipients Author: Irina B. Torres, Carla Burballa, José M. González-Posada, Domingo Hernández, Esteban Porrini, Janire Perurena, Vicente Cortina, Manel Perelló, Dolores Redondo-Pachón, Ana González-Rine, Mercedes Cabello, Maria José Pérez-Sáez, Marta Crespo, Oriol Bestard, Daniel Serón and Francesc Moreso Subject: Living kidney transplantation offers the best results for end-stage renal disease patients, but concerns about cardiovascular risk after nephrectomy for kidney donors have been raised. We aimed to estimate the contribution of renal function to endothelial dysfunction (ED) and subclinical inflammation in a non-interventional, prospective, multicenter, longitudinal study with two cohorts: living kidney donors and their transplant recipients (registered clinical trial NCT02515643). The measured glomerular filtration rate (mGFR) by iohexol clearance, estimated GFR according to the CKD-EPI and MDRD-4 formulas, and levels of endothelial dysfunction (sVCAM-1, sICAM-1, E-selectin, von Willebrand Factor, pentraxin, and urinary albumin-to-creatinine ratio) and subclinical inflammation biomarkers (sIL-6, sTNF-R1, sTNF-R2, sTWEAK, and high-sensitivity C-reactive protein) were determined at baseline and 1-year follow-up. Fifty pairs of donors and recipients were recruited between 2015 and 2018. Among the endothelial dysfunction biomarkers, sVCAM-1 increased in donors and decreased in recipients (p < 0.01) while, among the inflammation biomarkers, sTNFR1 and sTNFR2 significantly increased in donors and decreased in recipients (p < 0.001). After transplantation, parallel increases and decreases in ED and subclinical inflammation biomarkers were observed in the donor and recipient cohorts, respectively. Long-term follow-up is needed to characterize the cardiovascular risk associated with these changes. 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