352c89cb4a762de124b9a932a34440d7 fimmu-16-1648725.pdf 46e5f870cf8a9249ebfb15e7af492a640b8419a2 fimmu-16-1648725.pdf 5e74d61c82318a5c6c4e8a6aa7c5268e4ed2c3cd1f2702da0ec4a061a1402508 fimmu-16-1648725.pdf Title: Identification of cellular factors associated with inflammation and neurodegeneration in multiple sclerosis Subject: Rodero-Romero A, Fernández-Velasco JI, Monreal E, Sainz-Amo R, Álvarez-Lafuente R, Comabella M, Ramió-Torrentà L, García-Domínguez JM, Villarrubia N, Sainz de la Maza S, Domínguez-Mozo M, Quiroga-Varela A, Chico-García JL, Rodriguez-Jorge F, Veiga-Gonzalez JL, Roldán-Santiago E, Espiño M, Rodríguez-Martín E, Álvarez G, Masjuan J, Montalban X, Costa-Frossard L and Villar LM (2025) Identification of cellular factors associated with inflammation and neurodegeneration in multiple sclerosis. Front. Immunol. 16:1648725. doi: 10.3389/fimmu.2025.1648725 Keywords: multiple sclerosis; serum biomarkers; cellular phenotype and function; neurofilament light chain; glial fibrillary acidic protein; demyelinating disease of central nervous system Author: Alexander Rodero-Romero CreationDate: Tue Aug 5 05:08:43 2025 CEST ModDate: Tue Aug 5 05:08:47 2025 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.389 x 841.663 pts (A4) Page rot: 0 File size: 2526594 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DOHOPA+AdvTTd3a5f740 Type 1C WinAnsi yes yes no 540 0 DOHPAJ+AdvTT2c8ce45a Type 1C WinAnsi yes yes no 536 0 DOHPAK+AdvTTd168d80a.I Type 1C WinAnsi yes yes no 669 0 DOHPAL+AdvOT3f84ef53 Type 1C WinAnsi yes yes no 538 0 DOHOPB+AdvTTd3a5f740+fb Type 1C Custom yes yes yes 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Methods Destination: s2 Children: Item: Title: 2.1 Patients and controls Destination: s2_1 Item: Title: 2.2 Samples Destination: s2_2 Item: Title: 2.3 Monoclonal antibodies Destination: s2_3 Item: Title: 2.4 Labelling of surface molecules Destination: s2_4 Item: Title: 2.5 In vitro stimulation and intracellular cytokine staining of PBMCs Destination: s2_5 Item: Title: 2.6 Flow cytometry Destination: s2_6 Item: Title: 2.7 sNfL and sGFAP quantification Destination: s2_7 Item: Title: 2.8 Statistical analysis Destination: s2_8 Item: Title: 2.9 Data availability Destination: s2_9 Item: Title: 3 Results Destination: s3 Children: Item: Title: 3.1 Differences in peripheral blood immune cell subsets Destination: s3_1 Item: Title: 3.2 Differences in the immune cell subsets present in CSF Destination: s3_2 Item: Title: 4 Discussion Destination: s4 Item: Title: Data availability statement Destination: bmark_-1258588262 Item: Title: Ethics statement Destination: bmark_1370551237 Item: Title: Author contributions Destination: bmark_-1208584890 Item: Title: Funding Destination: bmark_1865464027 Item: Title: Acknowledgments Destination: bmark_-366698314 Item: Title: Conflict of interest Destination: bmark_1089982005 Item: Title: Generative AI statement Destination: bmark_-1453097031 Item: Title: Publisher’s note Destination: bmark_-1256977648 Item: Title: Supplementary material Destination: bmark_632582120 Item: Title: References Destination: bmark_2090415954 Info: Title: Identification of cellular factors associated with inflammation and neurodegeneration in multiple sclerosis Author: Alexander Rodero-Romero Subject: Rodero-Romero A, Fernández-Velasco JI, Monreal E, Sainz-Amo R, Álvarez-Lafuente R, Comabella M, Ramió-Torrentà L, García-Domínguez JM, Villarrubia N, Sainz de la Maza S, Domínguez-Mozo M, Quiroga-Varela A, Chico-García JL, Rodriguez-Jorge F, Veiga-Gonzalez JL, Roldán-Santiago E, Espiño M, Rodríguez-Martín E, Álvarez G, Masjuan J, Montalban X, Costa-Frossard L and Villar LM (2025) Identification of cellular factors associated with inflammation and neurodegeneration in multiple sclerosis. Front. 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García-Domínguez Noelia Villarrubia Susana Sainz de la Maza María Domínguez-Mozo Ana Quiroga-Varela Juan Luís Chico-García Fernando Rodriguez-Jorge José Luis Veiga-Gonzalez Ernesto Roldán-Santiago Mercedes Espiño Eulalia Rodríguez-Martín Gary Álvarez Jaime Masjuan Xavier Montalban Lucienne Costa-Frossard Luisa María Villar Rodero-Romero A, Fernández-Velasco JI, Monreal E, Sainz-Amo R, Álvarez-Lafuente R, Comabella M, Ramió-Torrentà L, García-Domínguez JM, Villarrubia N, Sainz de la Maza S, Domínguez-Mozo M, Quiroga-Varela A, Chico-García JL, Rodriguez-Jorge F, Veiga-Gonzalez JL, Roldán-Santiago E, Espiño M, Rodríguez-Martín E, Álvarez G, Masjuan J, Montalban X, Costa-Frossard L and Villar LM (2025) Identification of cellular factors associated with inflammation and neurodegeneration in multiple sclerosis. Front. Immunol. 16:1648725. doi: 10.3389/fimmu.2025.1648725 Copyright © 2025 Rodero-Romero, Fernández-Velasco, Monreal, Sainz-Amo, Álvarez-Lafuente, Comabella, Ramió-Torrentà, García-Domínguez, Villarrubia, Sainz de la Maza, Domínguez-Mozo, Quiroga-Varela, Chico-García, Rodriguez-Jorge, Veiga-Gonzalez, Roldán-Santiago, Espiño, Rodríguez-Martín, Álvarez, Masjuan, Montalban, Costa-Frossard and Villar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. multiple sclerosis serum biomarkers cellular phenotype and function neurofilament light chain glial fibrillary acidic protein demyelinating disease of central nervous system Identification of cellular factors associated with inflammation and neurodegeneration in multiple sclerosis multiple sclerosis; serum biomarkers; cellular phenotype and function; neurofilament light chain; glial fibrillary acidic protein; demyelinating disease of central nervous system True https://www.frontiersin.org/legal/copyright-statement 2025-08-05T11:08:47+08:00 2025-08-05T11:08:43+08:00 2025-08-05T11:08:47+08:00 uuid:23189541-785a-499a-b65c-9f9f490d3117 uuid:1613f204-d5eb-4e0d-a1d6-30b04956d6f0 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 221, 685, 525, 706 Annotation: Subtype: Link Rect: 221, 660, 505, 681 Annotation: Subtype: Link Rect: 221, 635, 473, 656 Annotation: Subtype: Link Rect: 221, 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