1fe1d11b4cf7a5882a222f4ea9edcd07 JMV-97-e70523.pdf 3e35a8cee68293dae263256c2b3fd516e0b1d582 JMV-97-e70523.pdf 85b4baf9019eb659bd726360a44070b49c4cfbc048f82acef95fdfa9356e7b93 JMV-97-e70523.pdf Title: A New Next-Generation Sequencing Approach in Human Cytomegalovirus for the Identification of Antiviral Resistance Mutations and Genotypic Classification CreationDate: Tue Jul 29 17:13:31 2025 CEST ModDate: Tue Jul 29 17:13:31 2025 CEST Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: AcroForm JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 939340 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BHJEKO+AdvOTa9c75b03.BI Type 1C WinAnsi yes yes no 366 0 BHJFCM+AdvOT5d4a5f24.B Type 1C WinAnsi yes yes no 367 0 BHJFDM+AdvOT5d4a5f24.B+20 Type 1C Custom yes yes yes 374 0 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mk:titleTitle1 Children: Item: Title: 1 Introduction Destination: mk:titleH12 Item: Title: 2 Materials and Methods Destination: mk:titleH13 Children: Item: Title: 2.1 Primer Design—Overlapping Amplicons Destination: mk:titleH24 Item: Title: 2.2 Enrichment Multiplex PCR Destination: mk:titleH25 Item: Title: 2.3 Library Preparation and Next-Generation Sequencing Destination: mk:titleH26 Item: Title: 2.4 Data Processing and Analysis Destination: mk:titleH27 Item: Title: 2.5 Variant Visualization Through Pileup Inspection Destination: mk:titleH28 Item: Title: 2.6 Repeatability Validation of the Technique for the Amplification of Resistance-Related Genes Destination: mk:titleH29 Item: Title: 2.7 Accuracy Validation by a Comparison between Sanger and NGS Destination: mk:titleH210 Item: Title: 2.8 Accuracy Validation Using an External Quality Control Destination: mk:titleH211 Item: Title: 2.9 Phylogenetic Analysis of UL55 (gB) Region Destination: mk:titleH212 Item: Title: 2.10 Measurement of the Limit of Detection (LoD) Destination: mk:titleH213 Item: Title: 3 Results Destination: mk:titleH114 Children: Item: Title: 3.1 Primer Design Destination: mk:titleH215 Item: Title: 3.2 Reproducibility Evaluation of Wild-Type Control Material Destination: mk:titleH216 Item: Title: 3.3 NGS Technique Versus Sanger Sequencing Destination: mk:titleH217 Item: Title: 3.4 Implementation With External Quality Controls Destination: mk:titleH218 Item: Title: 3.5 Genotyping of HCMV Based on the gB Region Destination: mk:titleH219 Item: Title: 3.6 Limit of Detection of the Technique Destination: mk:titleH220 Item: Title: 4 Discussion Destination: mk:titleH121 Item: Title: Author Contributions Destination: mk:titleH122 Item: Title: Acknowledgments Destination: mk:titleH123 Item: Title: Conflicts of Interest Destination: mk:titleH124 Item: Title: Data Availability Statement Destination: mk:titleH125 Item: Title: References Destination: mk:titleH126 Item: Title: Supporting Information Destination: 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