52514bd80e84b70f5d1f7954ea055d7f PhyPla_a2025_v177_n1.pdf feb8a32e0c6ebe2fd0d40527656de22a353b850a PhyPla_a2025_v177_n1.pdf a82423c66b2cf563baba76d89226a347a4f64cce6c4aaf0c13bbbda259ed07e9 PhyPla_a2025_v177_n1.pdf Title: MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering Subject: Physiologia Plantarum 2025.177:e70088 Creator: Arbortext Advanced Print Publisher 9.1.520/W Unicode Producer: Acrobat Distiller 24.0 (Windows); modified using iText 4.2.0 by 1T3XT CreationDate: Mon Jan 27 11:44:17 2025 CET ModDate: Tue Jun 23 16:31:19 2026 CEST Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 9052120 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JMDLGC+AdvTTa9c1b374+20 Type 1C WinAnsi yes yes no 109 0 JMDMBP+GillSansMT Type 1C WinAnsi yes yes no 52 0 JMDHMC+AdvTT6071803a.B Type 1C WinAnsi yes yes no 54 0 JMDJLH+AdvTT9c438aa4.BI Type 1C WinAnsi yes yes no 513 0 JMDJMI+AdvTT6071803a.B+20 Type 1C WinAnsi yes yes no 514 0 JMDLEP+AdvTTa9c1b374 Type 1C WinAnsi yes yes no 55 0 JMDLFA+AdvTTeb5f0e55.I Type 1C WinAnsi yes yes no 57 0 JMDLFB+AdvP4C4E74 Type 1C Custom yes yes yes 58 0 JMEFAK+AdvP4C4E59 Type 1C WinAnsi yes yes no 50 0 JMEFAL+AdvTTa9c1b374+03 Type 1C Custom yes yes yes 51 0 JMEEPJ+AdvOT8608a8d1+20 Type 1C WinAnsi yes yes no 53 0 JMEFAM+AdvTTa9c1b374+21 Type 1C WinAnsi yes yes no 56 0 Times-Roman Type 1 WinAnsi no no no 5 0 JMFJJP+AdvTTfac587ca.B Type 1C WinAnsi yes yes no 94 0 JMEIIH+AdvOT8608a8d1+03 Type 1C Custom yes yes yes 95 0 JMLBGF+AdvOTdd3b7348.I+20 Type 1C WinAnsi yes yes no 243 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-06-24 04:08:50 CEST RepresentationInformation: PhyPla_a2025_v177_n1.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-06-23 16:32:46 CEST Size: 9052120 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 2 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering Destination: atl_PPL_70088_ Children: Item: Title: Abstract Destination: HeadingPPL_70088ABSid_1 Item: Title: 1 | INTRODUCTION Destination: HeadingPPL_70088ppl70088-sec-0001 Item: Title: 2 | MATERIALS AND METHODS Destination: HeadingPPL_70088ppl70088-sec-0002 Children: Item: Title: 2.1 | Strains and growth conditions Destination: HeadingPPL_70088ppl70088-sec-0003 Item: Title: 2.2 | Molecular biology protocols Destination: HeadingPPL_70088ppl70088-sec-0004 Item: Title: 2.3 | Biobricks design and construction Destination: HeadingPPL_70088ppl70088-sec-0005 Item: Title: 2.4 | MoCloro Level 1 assembly reactions and generation of selection marker Destination: HeadingPPL_70088ppl70088-sec-0006 Item: Title: 2.5 | MoCloro Level 2 assembly reactions Destination: HeadingPPL_70088ppl70088-sec-0007 Item: Title: 2.6 | Construction of MoCloro Level 2 destination vectors pWF.K.4, pK4 and pLM20.K.4 Destination: HeadingPPL_70088ppl70088-sec-0008 Item: Title: 2.7 | Plasmid quality control Destination: HeadingPPL_70088ppl70088-sec-0009 Item: Title: 2.8 | Chlamydomonas chloroplast transformation by particle microbombardment Destination: HeadingPPL_70088ppl70088-sec-0010 Item: Title: 2.9 | Chlamydomonas genotyping by PCR Destination: HeadingPPL_70088ppl70088-sec-0011 Item: Title: 2.10 | GUS assay Destination: HeadingPPL_70088ppl70088-sec-0012 Item: Title: 2.11 | Protein quantification from the extracts by BCA Destination: HeadingPPL_70088ppl70088-sec-0013 Item: Title: 2.12 | Protein extraction and immunoblot analysis of transplastomic lines Destination: HeadingPPL_70088ppl70088-sec-0014 Item: Title: 3 | RESULTS & DISCUSSION Destination: HeadingPPL_70088ppl70088-sec-0015 Children: Item: Title: 3.1 | Construction of standardized Level 0 biobricks and Level 2 destination vector for Chlamydomonas chloroplast transfo... Destination: HeadingPPL_70088ppl70088-sec-0016 Item: Title: 3.2 | Generation of combinatorial constructs for functional testing of biobricks Destination: HeadingPPL_70088ppl70088-sec-0017 Item: Title: 3.3 | Testing multigene construction and expression Destination: HeadingPPL_70088ppl70088-sec-0018 Item: Title: 3.4 | Construction of a new vector to enable new integration sites in Chlamydomonas plastome Destination: HeadingPPL_70088ppl70088-sec-0019 Item: Title: 4 | CONCLUSIONS Destination: HeadingPPL_70088ppl70088-sec-0020 Item: Title: AUTHOR CONTRIBUTIONS Destination: HeadingPPL_70088ppl70088-sec-0021 Item: Title: ACKNOWLEDGEMENTS Destination: HeadingPPL_70088ppl70088-sec-0022 Item: Title: DATA AVAILABILITY STATEMENT Destination: HeadingPPL_70088ppl70088-sec-0024 Item: Title: ORCID Destination: HeadingPPL_70088ORCid Item: Title: REFERENCES Destination: HeadingPPL_70088ppl70088-bibl-00011 Item: Title: SUPPORTING INFORMATION Destination: HeadingPPL_70088SUPPid Filters: FilterPipeline: 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