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Title:           A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants in Chlamydomonas reinhardtii
Subject:         MethodsX, 15 (2025) 103416. doi:10.1016/j.mex.2025.103416
Keywords:        "CRISPR-Cas9 protocol"; "Chlamydomonas"; "Microalgae"; "Indel detection, knockout mutants"; "Simple and quick"
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      Title: A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants in Chlamydomonas reinhardtii
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       Item: 
        Title: Background
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         Item: 
          Title: Description of protocol
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         Item: 
          Title: Step 1 - gRNA and primer design
          Destination: subsection..1.2
         Item: 
          Title: Step 2: primer test
          Destination: subsection..1.3
         Item: 
          Title: Step 3: Cas9 in vitro cleavage test
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           Item: 
            Title: Prior to transformation
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         Item: 
          Title: Step 4: Transformation
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         Item: 
          Title: Assembly of the CRISPR-Cas9 machinery
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         Item: 
          Title: Step 5: Screening for large-insertion and short-indel mutants
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         Item: 
          Title: Protocol validation
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         Item: 
          Title: Troubleshooting
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         Item: 
          Title: Limitations
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        Title: Acknowledgements
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       Item: 
        Title: Declaration of competing interest
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       Item: 
        Title: Supplementary materials
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       Item: 
        Title: References
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  Checksum: 0636e3e4
   Type: CRC32
  Checksum: c5af092d275c15f9be5dffdc94e8bf9b
   Type: MD5
  Checksum: 98a147e66a6dffcca939ac816eaadcdc99549659
   Type: SHA-1
  Checksum: 8023913965a3285e0540c00b0635b8179234d4c37a7f3254f882a72683f4ee7d
   Type: SHA-256