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Title:           Targeted Editing and Phenotypic Profiling of CmOFP13 Mutants Reveal Its Role in Melon Fruit Morphogenesis
Subject:         Physiologia Plantarum 2025.177:e70641
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    Outlines: 
     Item: 
      Title: Targeted Editing and Phenotypic Profiling of CmOFP13 Mutants Reveal Its Role in Melon Fruit Morphogenesis
      Children: 
       Item: 
        Title: ABSTRACT
       Item: 
        Title: 1   |   Introduction
       Item: 
        Title: 2   |   Material and Methods
        Children: 
         Item: 
          Title: 2.1   |   Guide RNA Design and Cloning
         Item: 
          Title: 2.2   |   Melon Transformation and In Vitro Culture
         Item: 
          Title: 2.3   |   PCR and Sequencing
         Item: 
          Title: 2.4   |   Genetic and Protein Analysis
         Item: 
          Title: 2.5   |   Plant Growth
         Item: 
          Title: 2.6   |   Fruit Phenotyping
         Item: 
          Title: 2.7   |   Ovary Development Analysis
         Item: 
          Title: 2.8   |   Ovary Cell Imaging and Analysis
         Item: 
          Title: 2.9   |   Statistical Analyses
       Item: 
        Title: 3   |   Results
        Children: 
         Item: 
          Title: 3.1   |   Generation of CRISPR/Cas9 CmOFP13 Mutants
         Item: 
          Title: 3.2   |   From Tetraploid to Diploid Background
         Item: 
          Title: 3.3   |   Putative Effect of the Mutation in the CmOFP13 Protein and Phylogenetic Analysis
         Item: 
          Title: 3.4   |   Phenotyping of the Gene-Edited Plants
       Item: 
        Title: 4   |   Discussion
        Children: 
         Item: 
          Title: 4.1   |   Polyploidy Affects Fruit Shape in Melon, Which Can Be Overcome by Crossing
         Item: 
          Title: 4.2   |   CmOPF13 Is a Regulator of Ovary and Fruit Shape in Melon
         Item: 
          Title: 4.3   |   OVATE Domain of CmOFP13 Is Not Essential for Plant Development
         Item: 
          Title: 4.4   |   Phylogenetical Analyses Reveal New Candidate Genes to Study Melon Shape
       Item: 
        Title: 5   |   Conclusions
       Item: 
        Title: Author Contributions
       Item: 
        Title: Acknowledgments
       Item: 
        Title: Funding
       Item: 
        Title: Disclosure
       Item: 
        Title: Data Availability Statement
       Item: 
        Title: References
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  Checksum: a852b6f9
   Type: CRC32
  Checksum: dcf552df3975304c4bc36a1962ca79fc
   Type: MD5
  Checksum: 9cb8a4ff0f70a1ff0f1b1059a9472f6921574624
   Type: SHA-1
  Checksum: 4b8fb4d0bc9f1f25317c4c276f1c9b217a198314765e685177129f92cd673d72
   Type: SHA-256