2f01bcab5ea5f1eaf1675b8ff63dc0d0 THEW_a2025n6p100125.pdf d637bc5f72341ac9a9e8b50dc7310c231be9e211 THEW_a2025n6p100125.pdf 635fab64d8ee1cc2de80ebdeb2aaa984afcdf794249618d63c8f54637c90ac8d THEW_a2025n6p100125.pdf Title: Follicular fluid supports cumulus expansion in post-mortem recovered cumulus-oocyte complexes of Hippopotamus amphibius Subject: Theriogenology Wild, 6 (2025) 100125. doi:10.1016/j.therwi.2025.100125 Keywords: Hipopotamus amphibius,In vitro maturation,Cumulus expansion,Follicular fluid,Post-mortem,Cryobank Author: Judith Diaz-Muñoz Creator: Elsevier Producer: Acrobat Distiller 8.1.0 (Windows) CreationDate: Wed Mar 19 14:06:05 2025 CET ModDate: Thu Mar 20 06:41:12 2025 CET Custom Metadata: yes Metadata Stream: yes Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 793.701 pts Page rot: 0 File size: 3396653 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID 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LastModified: 2026-07-29 07:01:31 CEST Size: 3396653 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Tagged PDF PDFMetadata: Objects: 937 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: true NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: English Outlines: Item: Title: Follicular fluid supports cumulus expansion in post-mortem recovered cumulus-oocyte complexes of Hippopotamus amphibius Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Chemicals and suppliers Destination: mk:H2_1 Item: Title: 2.2 Post-mortem recovery of the ovaries Destination: mk:H2_2 Item: Title: 2.3 Macroscopic evaluation Destination: mk:H2_3 Item: Title: 2.4 Microscopic evaluation Destination: mk:H2_4 Item: Title: 2.5 Follicular fluid aspiration and oocyte recovery Destination: mk:H2_5 Item: Title: 2.6 In vitro maturation Destination: mk:H2_6 Item: Title: 2.7 Cumulus cell expansion Destination: mk:H2_7 Item: Title: 2.8 Polar body extrusion Destination: mk:H2_8 Item: Title: 2.9 Statistical analysis Destination: mk:H2_9 Item: Title: 3 Results Destination: mk:H1_3 Children: Item: Title: 3.1 Macroscopic examination and follicular fluid collection Destination: mk:H2_10 Item: Title: 3.2 Microscopic examination Destination: mk:H2_11 Item: Title: 3.3 Cumulus cell expansion Destination: mk:H2_12 Item: Title: 3.4 Polar nuclear extrusion Destination: mk:H2_13 Item: Title: 4 Discussion Destination: mk:H1_4 Item: Title: 5 Conclusions Destination: mk:H1_5 Item: Title: Ethics approval and consent to participate Destination: mk:H1_6 Item: Title: Consent for publication 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