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Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Microbial culture and culture protocol Destination: mk:H2_1 Item: Title: 2.2 PHF-HUR methodology Destination: mk:H2_2 Children: Item: Title: 2.2.1 Setup configuration Destination: mk:H3_3 Item: Title: 2.2.2 PHF-HUR tests protocol Destination: mk:H3_4 Item: Title: 2.2.3 Data treatment and modelling Destination: mk:H3_5 Item: Title: 2.3 Traditional serum bottle test Destination: mk:H2_6 Item: Title: 2.4 Analytical methods Destination: mk:H2_7 Item: Title: 3 Results and discussion Destination: mk:H1_3 Children: Item: Title: 3.1 Abiotic characterization of the setup Destination: mk:H2_8 Item: Title: 3.2 Pressure effect on biological activity Destination: mk:H2_9 Item: Title: 3.3 Comparison with the conventional SBT methodology Destination: mk:H2_10 Item: Title: 3.4 Temperature effect on biological activity Destination: mk:H2_11 Item: Title: 3.5 Determination of the half-saturation constant and kinetic model comparison Destination: mk:H2_12 Item: Title: 4 Conclusions Destination: mk:H1_4 Item: Title: Funding information Destination: mk:H1_5 Item: Title: CRediT authorship contribution statement Destination: mk:H1_6 Item: Title: Declaration of competing interest Destination: mk:HH1_2 Item: Title: Acknowledgement Destination: mk:HH1_3 Item: Title: Supplementary materials Destination: mk:H1_7 Item: Title: Data availability Destination: datalink4 Item: Title: References Destination: mk:HH2_1 Info: Title: Assessing the temperature effect on the kinetics of a hydrogenotrophic methanogenic culture using a novel headspace-free methodology Author: Manuel Fachal-Suárez Subject: Chemical Engineering Journal Advances, 24 (2025) 100951. doi:10.1016/j.ceja.2025.100951 Keywords: Hydrogenotrophic methanogenesis,Activity assessment,Temperature effect,Hydrogen uptake rate,Half-saturation constant,Kinetic modelling 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Chemical Engineering Journal Advances, 24 (2025) 100951. doi:10.1016/j.ceja.2025.100951 Hydrogenotrophic methanogenesis Activity assessment Temperature effect Hydrogen uptake rate Half-saturation constant Kinetic modelling Assessing the temperature effect on the kinetics of a hydrogenotrophic methanogenic culture using a novel headspace-free methodology Manuel Fachal-Suárez Àlex Gaona Pablo Benavides Javier Lafuente Marc A. Deshusses Daniel Gonzalez David Gabriel VoR 28th November 2025 Acrobat Distiller 8.1.0 (Windows) Hydrogenotrophic methanogenesis,Activity assessment,Temperature effect,Hydrogen uptake rate,Half-saturation constant,Kinetic modelling 28th November 2025 sciencedirect.com elsevier.com true 2010-04-23 10.1016/j.ceja.2025.100951 noindex journal © 2025 The Author(s). 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