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Title:           Five centuries of lake deoxygenation and microbial shifts revealed by sedimentary DNA
Subject:         Anthropocene, 53 (2026) 100504. doi:10.1016/j.ancene.2025.100504
Keywords:        Anthropocene,Climate change,Community responses,Hypoxia,Hemp retting,Functional resilience
Author:          Teresa Vegas-Vilarrúbia
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      Title: Five centuries of lake deoxygenation and microbial shifts revealed by sedimentary DNA
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        Title: 1 Introduction
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       Item: 
        Title: 2 Materials and methods
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         Item: 
          Title: 2.1 Study site
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         Item: 
          Title: 2.2 Coring, dating, geochemical (XRF) analyses, and lake volume calculations
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         Item: 
          Title: 2.3 Analysis of sedimentary microbial communities
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         Item: 
          Title: 2.4 Environmental descriptors
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          Title: 2.5 Statistical analyses
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           Item: 
            Title: 2.5.1 Alpha and beta diversity of filtered microbial communities
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           Item: 
            Title: 2.5.2 Percentage diagram of the stratigraphically constrained ASVs
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           Item: 
            Title: 2.5.3 Multivariate analyses
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        Title: 3 Results
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         Item: 
          Title: 3.1 Variations in the volume of anoxic water
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         Item: 
          Title: 3.2 Composition of sedimentary bacterial communities
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         Item: 
          Title: 3.3 Variation in microbial ASVs along the stratigraphic record
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           Item: 
            Title: 3.3.1 Zone M-1 (14 samples): depth 970–890 mm, circa 1512–1636 CE
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           Item: 
            Title: 3.3.2 Zone M-2 (12 samples): depth 885–820 mm, circa 1637–1719 CE
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           Item: 
            Title: 3.3.3 Zone M-3 (1 sample): 815–805 mm, circa 1720–1737 CE
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           Item: 
            Title: 3.3.4 Zone M-4 (15 samples): depth 800–625 mm, circa 1738–1840 CE
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           Item: 
            Title: 3.3.5 Zone M-5 (13 samples): depth 620–205 mm, circa 1841–1912 CE
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           Item: 
            Title: 3.3.6 Zone M-6 (10 samples): depth 195–140 mm, circa 1912–1963
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            Title: 3.3.7 Zone M-7 (7 samples): depth of 135–85 mm, circa 1963–2006
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          Title: 3.4 Multivariate analysis
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        Title: 4 Discussion
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          Title: 4.1 Dynamics of microbial communities, oxygenation phases and environmental drivers
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           Item: 
            Title: 4.1.1 Early anoxia, episodic oxygenation and the onset of Cannabis retting (XVIth and XVIIth centuries)
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            Title: 4.1.2 Frequent mixing events, intensification of Cannabis retting, and sulfate reduction (XVIIth and XVIIIth centuries)
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            Title: 4.1.3 Episodic sediment influx, nutrient pulses and the influence of the retting (XVIIIth century)
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            Title: 4.1.4 Decline of retting, sulfate reduction and onset of euxinic conditions (XVIIIth and XIXth centuries)
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            Title: 4.1.5 Turbidites and cessation of retting activities (XIXth to early XXth centuries)
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            Title: 4.1.6 Thermal stratification, reforestation, and internal productivity (Late XXth and XXIth centuries)
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            Title: 4.1.7 Intensification of climate warming and the strengthening of stratification
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          Title: 4.2 Long-term deoxygenation dynamics and resilience of the bacterial community
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          Title: 4.3 Long-term deoxygenation trends in context
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        Title: 5 Conclusions
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       Item: 
        Title: CRediT authorship contribution statement
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       Item: 
        Title: Declaration of Competing Interest
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        Title: Acknowledgements
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        Title: Appendix A Supporting information
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       Item: 
        Title: Data availability
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        Title: References
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  Checksum: c781c5f4
   Type: CRC32
  Checksum: f5760e07ed97d740fe0141958b166f44
   Type: MD5
  Checksum: f6cf9eb87319d0a9d57f5d88deb7b1bce4f850d7
   Type: SHA-1
  Checksum: e1c1a2d44746d6afc651a7bf2eb56008ba3128ecc633646693bcccf60eb289f5
   Type: SHA-256