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Title:           Optimizing RT-qPCR for sex-specific analysis of iron-induced retinal gene expression
Subject:         Experimental Eye Research, 262 (2026) 110712. doi:10.1016/j.exer.2025.110712
Keywords:        Iron overload,Sex-variation,Gene expression,Reference genes,Housekeeping,RT-qPCR,Mouse,Retina,Retinal dysmorphism,Sex bias
Author:          Joan-Feliu Calafat
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      Title: Optimizing RT-qPCR for sex-specific analysis of iron-induced retinal gene expression
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        Title: 1 Introduction
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        Title: 2 Material and methods
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          Title: 2.1 Mice
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          Title: 2.2 Iron dextran injection
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          Title: 2.3 Retinal RNA isolation and cDNA synthesis
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          Title: 2.4 Selection of reference genes, primer design and efficiency
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          Title: 2.5 RT-qPCR
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          Title: 2.6 Data analysis
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          Title: 2.7 Validation of candidate reference genes
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          Title: 2.8 Statistical analysis
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        Title: 3 Results
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          Title: 3.1 Selected candidate reference genes and its RT-qPCR primer amplification efficiencies
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          Title: 3.2 Descriptive statistics of candidate reference genes
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          Title: 3.3 Stability test of candidate reference genes
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          Title: 3.4 Influence of reference gene selection on the retinal expression analysis of iron-regulated genes
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        Title: Declaration of competing interest
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    Author: Joan-Feliu Calafat
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    Keywords: Iron overload,Sex-variation,Gene expression,Reference genes,Housekeeping,RT-qPCR,Mouse,Retina,Retinal dysmorphism,Sex bias
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  Checksum: 17736791
   Type: CRC32
  Checksum: 9303911e434c16b2cd1a3b061c77bfeb
   Type: MD5
  Checksum: 5e8a2b1fc1bd3055c3e7fada1af4d936ad103121
   Type: SHA-1
  Checksum: 0f65136567ddddf5abdc6359d02d27fa5087b80a6332cf677fcb1669964fcbe2
   Type: SHA-256