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Title:           Evaluation of urinary amylase to creatinine ratio alone and in combination with urinary electrophoresis as a marker of renal damage in dogs with leishmaniosis undergoing conventional anti-Leishmania treatment
Subject:         Research in Veterinary Science, 206 (2026) 106160. doi:10.1016/j.rvsc.2026.106160
Keywords:        Creatinine,Mixed proteinuria,Sodium dodecyl-sulphate agarose gel,Urinary protein-to-creatinine ratio
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        Title: 1 Introduction
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        Title: 2 Material and methods
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          Title: 2.1 Dogs
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          Title: 2.2 Blood pressure measurement, sample collection and grouping
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          Title: 2.3 Treatment protocol
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          Title: 2.4 Blood tests
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            Title: 2.4.1 Leishmania serology
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          Title: 2.5 Urine examination
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          Title: 2.6 Urinary electrophoresis
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            Title: 2.6.1 Sample selection and SDS-AGE procedure
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            Title: 2.6.2 Gel interpretation and proteinuria patterns
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          Title: 2.7 Evaluation of Leishmania parasitic load
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            Title: 2.7.1 Bone marrow collection and sampling procedure
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            Title: 2.7.2 DNA extraction, real time-PCR protocol and interpretation
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          Title: 3.1 Clinical data of healthy dogs and dogs with leishmaniosis at diagnosis
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          Title: 3.2 Serum and urinary markers in healthy dogs and dogs with leishmaniosis at diagnosis
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          Title: 3.3 Serum and urinary markers in dogs with leishmaniosis post-treatment
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          Title: 3.4 Urinary electrophoresis and uAm/Cr in dogs with leishmaniosis at diagnosis and post-treatment
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          Title: 3.5 Relationship between uAm/Cr and various parameters in healthy dogs and dogs with leishmaniosis at diagnosis
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        Title: Appendix A Supplementary data
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