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(Rel. 1.28.0, 2023-05-18) Date: 2026-06-12 04:56:56 CEST RepresentationInformation: natcom_a2026m1v17p2132.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-06-11 17:27:53 CEST Size: 4056683 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 975 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: EN Outlines: Item: Title: Structural basis for transcriptional regulation by the cell division regulator MraZ in Mycoplasma genitalium Destination: title_link Children: Item: Title: Results Destination: A1 Children: Item: Title: MraZ binds to a highly conserved sequence upstream of its promoter Destination: B1 Item: Title: Oligomeric structural assemblies of MraZ Destination: B2 Item: Title: MraZ structure in complex with its cognate DNA promoter Destination: B3 Item: Title: DNA binding by the MraZ “cradle-like” structural motif Destination: B4 Item: Title: MraZ octamer bound to one box of the cognate promoter Destination: B5 Item: Title: MraZ oligomerization influences the DNA binding affinity Destination: B6 Item: Title: Discussion Destination: A2 Item: Title: Methods Destination: A3 Children: Item: Title: Plasmids, cloning and point mutation Destination: B7 Item: Title: Protein expression and purification Destination: B8 Item: Title: Electron microscopy (Negative staining) Destination: B9 Item: Title: Crystallization and data collection Destination: B10 Item: Title: Structure determination and refinement (X-ray crystallography) Destination: B11 Item: Title: CryoEM sample preparation and Data Collection Destination: B12 Item: Title: Structure determination and refinement (CryoEM) Destination: B13 Item: Title: Electrophoretic mobility shift assay Destination: B14 Item: Title: Reporter Assay Destination: B15 Item: Title: Transcription start site (TSS) determination Destination: B16 Item: Title: RNA extraction 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