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Title:           Optimizing solid-state fermentation of Trichoderma harzianum in packed-bed bioreactors using beer spent grains and wheat straw for the production of metabolites related to phosphorus availability
Subject:         Bioresource Technology, 456 (2026) 134917. doi:10.1016/j.biortech.2026.134917
Keywords:        Phosphate-solubilizing microorganisms,Agro-industrial residues,Fungal cultivation,Biostimulants,Multifunctional bio-inputs,Bioprocess scale-up
Author:          Natalia Alvarez Rodrigues
Creator:         Elsevier
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      Title: Optimizing solid-state fermentation of Trichoderma harzianum in packed-bed bioreactors using beer spent grains and wheat st ...
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        Title: 1 Introduction
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        Title: 2 Material and methods
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          Title: 2.1 Microorganisms and solid substrates
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          Title: 2.2 Solid-state fermentation
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           Item: 
            Title: 2.2.1 Evaluation of P release in flasks
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           Item: 
            Title: 2.2.2 Optimization of spore production in 0.5 ​L PBB
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            Title: 2.2.3 Time course analysis under optimized conditions in 0.5 ​L PBB
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           Item: 
            Title: 2.2.4 Process scale-up to a 22 ​L PBB
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            Title: 2.2.5 Assessment of oxygen consumption
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          Title: 2.3 Analytical procedures
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            Title: 2.3.1 Spore counting
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            Title: 2.3.2 Soluble and total P determination
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            Title: 2.3.3 Phosphatase activity assays
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            Title: 2.3.4 Quantification of indole derivatives
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            Title: 2.3.5 Quantification of total flavonoids
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            Title: 2.3.6 Air-filled porosity
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          Title: 3.1 Effect of the addition of alcohols on P release
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          Title: 3.2 Optimization of spore production in 0.5 ​L PBB
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          Title: 3.3 Time-course analysis of sporulation and associated metabolic responses in 0.5 ​L PBBs
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          Title: 3.4 Scale-up: 22 ​L packed bed bioreactor
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        Title: CRediT authorship contribution statement
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        Title: Declaration of competing interest
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        Title: Acknowledgements
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       Item: 
        Title: Data availability
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        Title: References
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    Keywords: Phosphate-solubilizing microorganisms,Agro-industrial residues,Fungal cultivation,Biostimulants,Multifunctional bio-inputs,Bioprocess scale-up
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