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Title:           Isolation and characterization of seminal extracellular vesicles subsets and their impact on sperm freezability in stallions
Subject:         Animal Reproduction Science, 289 (2026) 108160. doi:10.1016/j.anireprosci.2026.108160
Keywords:        Horse,Extracellular vesicles,Seminal plasma,Sperm,Cryopreservation
Author:          Isabel Barranco
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      Title: Isolation and characterization of seminal extracellular vesicles subsets and their impact on sperm freezability in stallions
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        Title: 1 Introduction
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        Title: 2 Material and methods
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          Title: 2.1 Animals and ejaculates
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          Title: 2.2 Seminal plasma isolation
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          Title: 2.3 Isolation of sEVs
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          Title: 2.4 Characterization of sEV subsets
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           Item: 
            Title: 2.4.1 Assessment of total protein concentration
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            Title: 2.4.2 Analysis of particle size distribution
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            Title: 2.4.3 Assessment of the particle and sEV concentrations
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            Title: 2.4.4 Evaluation of sEV morphology
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            Title: 2.4.5 Assessment of the presence of canonical EV-markers
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          Title: 2.6 Assessment of sperm characteristics
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          Title: 3.1 Sperm cryotolerance between GFE and PFE ejaculates
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          Title: 3.2 Characterization of sEVs subsets and differences between GFE and PFE
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        Title: CRediT authorship contribution statement
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        Title: Funding
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        Title: Declaration of Competing Interest
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        Title: Data availability
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    Author: Isabel Barranco
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    Keywords: Horse,Extracellular vesicles,Seminal plasma,Sperm,Cryopreservation
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   Type: SHA-1
  Checksum: b7b9705979ee2ea4970e60f9dbfdbe4d34ebac04e6e74603c95243d3379cded8
   Type: SHA-256