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Title:           Temperature and growth media affect conidia production, germination and virulence of Metarhizium spp. during simulated solid substrate fermentation conditions
Subject:         Fungal Biology, 130 (2026) 101794. doi:10.1016/j.funbio.2026.101794
Keywords:        Thermotolerant fungi,Conidia production,Conidia germination,Virulence,Tenebrio molitor,Principal component analysis
Author:          Arnau Sala
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      Title: Temperature and growth media affect conidia production, germination and virulence of Metarhizium spp. during simulated soli ...
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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         Item: 
          Title: 2.1 Fungal strains
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          Title: 2.2 Media preparation
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          Title: 2.3 Fungal growth and sporulation on solid substrates
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          Title: 2.4 Conidia suspensions and conservation
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          Title: 2.5 Quantifying conidia production and germination using oCelloScope
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           Item: 
            Title: 2.5.1 Preparation and software
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            Title: 2.5.2 Conidia counting
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            Title: 2.5.3 Conidia germination
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          Title: 2.6 Infection bioassays against insects
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           Item: 
            Title: 2.6.1 Insect culture
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            Title: 2.6.2 Insect bioassays set-up
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            Title: 2.6.3 Dose-response of two Metarhizium strains
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            Title: 2.6.4 Comparative virulence of conidia produced at different media and temperature conditions
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          Title: 2.7 Statistical analysis
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            Title: 2.7.1 OCelloScope assays
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            Title: 2.7.2 Insect survival assays
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            Title: 2.7.3 Multivariate analysis
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        Title: 3 Results
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          Title: 3.1 Effect of temperature, media and time conditions on conidia production and germination of thermotolerant M. strains
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           Item: 
            Title: 3.1.1 Constant (CT) and variable (VT) temperature experiments with M. brunneum EAMa 09/01-Su
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           Item: 
            Title: 3.1.2 Constant (CT) and variable (VT) temperature experiments with M. robertsii EAMa 01/158-Su
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          Title: 3.2 Effect of production conditions on virulence against T. molitor
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            Title: 3.2.1 Dose-response experiments on T. molitor larvae
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            Title: 3.2.2 Virulence of M. brunneum EAMa 09/01-Su against T. molitor larvae and adults
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            Title: 3.2.3 Virulence of M. robertsii EAMa 01/158-Su against T. molitor larvae
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          Title: 3.3 Thermotolerant strains relation with germination and conidia production
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        Title: 4 Discussion
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        Title: 5 Conclusions
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       Item: 
        Title: CRediT authorship contribution statement
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       Item: 
        Title: Declaration of competing interest
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        Title: Acknowledgments
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       Item: 
        Title: Appendix A Supplementary data
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        Title: References
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    Author: Arnau Sala
    Subject: Fungal Biology, 130 (2026) 101794. doi:10.1016/j.funbio.2026.101794
    Keywords: Thermotolerant fungi,Conidia production,Conidia germination,Virulence,Tenebrio molitor,Principal component analysis
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   Type: CRC32
  Checksum: 61af0966c068e0ed232f7d5b9e133523
   Type: MD5
  Checksum: aa44fe7a7116dfcc940b7742a3449184c9990522
   Type: SHA-1
  Checksum: 6e575e0bfc29b8377eec2cd9128ec7b7a491442476eb1491843be50f1f4fd139
   Type: SHA-256