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Title:           Extracellular vesicles from small-follicle follicular fluid improve redox balance and embryo development in naturally heat-stressed bovine oocytes
Subject:         Theriogenology, 264 (2026) 118041. doi:10.1016/j.theriogenology.2026.118041
Keywords:        Cattle,Oxidative stress,Oocyte competence,Hot season,Exosome
Author:          Judith Diaz-Muñoz
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      Title: Extracellular vesicles from small-follicle follicular fluid improve redox balance and embryo development in naturally heat- ...
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 Chemicals and suppliers
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          Title: 2.2 Experimental design
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          Title: 2.3 FF collection and FF-EVs isolation
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          Title: 2.4 EVs characterization
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            Title: 2.4.1 NTA
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            Title: 2.4.2 Cryo-EM
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            Title: 2.4.3 Flow cytometry
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          Title: 2.5 EVs staining and uptake by cumulus-oocyte complexes
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          Title: 2.6 Oocyte collection and in vitro maturation, fertilization, and embryo culture
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          Title: 2.7 Levels of reactive oxygen species and glutathione
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          Title: 2.8 TUNEL detection of fragmented oocyte DNA
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          Title: 2.9 Differential cell count and apoptosis staining of blastocysts
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          Title: 2.10 Lipid content
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          Title: 2.11 Statistical analysis
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        Title: 3 Results
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          Title: 3.1 Characterization of FF-EVs from large and small follicles
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          Title: 3.2 FF-EVs uptake by COCs
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          Title: 3.3 Oxidative stress in IVM naturally heat-stressed bovine oocytes supplemented with FF-EVs from small or large follicles
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          Title: 3.4 DNA fragmentation in IVM naturally heat-stressed bovine oocytes supplemented with FF-EVs from small or large follicles
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          Title: 3.5 Embryo development in IVM naturally heat-stressed bovine oocytes supplemented FF-EVs from small or large follicles
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          Title: 3.6 Differential cell count and apoptosis of blastocysts derived from naturally heat-stressed summer bovine oocytes supplem ...
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          Title: 3.7 Oxidative stress levels in blastocysts derived from naturally heat-stressed bovine oocytes supplemented with FF-EVs fro ...
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          Title: 3.8 Lipid content in blastocysts derived from naturally heat-stressed bovine oocytes supplemented with FF-EVs from small or ...
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        Title: 5 Conclusions
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        Title: Ethics approval and consent to participate
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        Title: Consent for publication
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        Title: Availability of data and materials
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        Title: Funding
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        Title: CRediT authorship contribution statement
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        Title: Declaration of competing interests
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        Title: LIST OF ABBREVIATIONS
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       Item: 
        Title: Appendix A Supplementary data
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        Title: References
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    Subject: Theriogenology, 264 (2026) 118041. doi:10.1016/j.theriogenology.2026.118041
    Keywords: Cattle,Oxidative stress,Oocyte competence,Hot season,Exosome
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      Annotation: 
       Subtype: Link
       Rect: 355, 437, 486, 443
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 522, 413, 555, 419
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 323, 405, 447, 411
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 415, 381, 533, 388
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 483, 365, 555, 372
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 323, 357, 360, 364
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 510, 341, 555, 348
       Flags: Print
      Annotation: 
       Subtype: Link
       Rect: 323, 333, 395, 340
       Flags: Print
  Checksum: 87c3a3bd
   Type: CRC32
  Checksum: 78cc84ee783f09890ac095e560d2cf06
   Type: MD5
  Checksum: 31cb2bdbd4cadf9a8d69dfe4d06fd9d0babdd6c2
   Type: SHA-1
  Checksum: a7ed6dd9180a84189dd54978918173c4de1b01fd29b2f88593e7bf3842c7dd95
   Type: SHA-256