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Microbiol. 17:1887353. doi: 10.3389/fmicb.2026.1887353 Author: Vanessa Pleguezuelos Creator: Adobe InDesign 19.5 (Windows) Producer: Adobe PDF Library 17.0; modified using iTextSharp™ 5.5.13.3 ©2000-2022 iText Group NV (AGPL-version) CreationDate: Fri Jul 24 14:31:25 2026 CEST ModDate: Fri Jul 24 14:31:41 2026 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 605471 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FSENSD+MuseoSans-500 Type 1C Custom yes yes yes 1633 0 DPFBQH+MuseoSans-700 Type 1C WinAnsi yes yes yes 1643 0 BMBPOL+MuseoSans-300 Type 1C Custom yes yes yes 1644 0 ZJCDMP+MuseoSans-300Italic Type 1C WinAnsi yes yes yes 1651 0 XGDRKT+MuseoSans-500Italic Type 1C WinAnsi yes yes yes 1652 0 XGDRKT+STIXGeneral-Italic Type 1C Custom yes yes yes 1662 0 NRDEAN+STIXGeneral-Regular Type 1C Custom yes yes yes 1663 0 PCAQCJ+MuseoSans-700Italic Type 1C WinAnsi yes yes yes 1664 0 OYQKOL+MinionPro-Regular Type 1C Custom yes yes yes 113 0 DDRHQH+MinionPro-It Type 1C Custom yes yes yes 108 0 TMQUGB+STIX-Regular Type 1C WinAnsi yes yes yes 104 0 RJRIEF+MinionPro-Bold Type 1C WinAnsi yes yes yes 119 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-09-30 04:02:22 CEST RepresentationInformation: FRIM_a2026v17m07.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-09-29 05:22:57 CEST Size: 605471 Format: PDF Version: 1.6 Status: Well-Formed, but not valid SignatureMatches: PDF-hul ErrorMessage: Annotation object is not a dictionary ID: PDF-HUL-114 Offset: 81478 MIMEtype: application/pdf PDFMetadata: Objects: 1698 FreeObjects: 1428 IncrementalUpdates: 2 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Language: en-US Outlines: Item: Title: Impact of microbiological testing protocols on discard rates in donor human milk with a focus on Bacillus cereus Children: Item: Title: 1 Introduction Item: Title: 2 Materials and methods Children: Item: Title: 2.1 Study setting and design Item: Title: 2.2 Donor screening, milk collection, transport and handling Item: Title: 2.3 Pre-pasteurization microbiological testing Item: Title: 2.4 Post-pasteurization microbiological testing Item: Title: 2.5 Bacillus cereus sensu lato pre-pasteurization and post-pasteurization concordance Item: Title: 2.6 Statistical analysis Item: Title: 3 Results and discussion Children: Item: Title: 3.1 Total viable aerobic microbial count of pre-pasteurization donor human milk Item: Title: 3.2 Specific microbial count of pre-pasteurization donor human milk Item: Title: 3.3 Pathogen prevalence in pre-pasteurization donor human milk: methodological and seasonal determinants Item: Title: 3.4 Pre-pasteurization donor human milk rejection rates Item: Title: 3.5 Post-pasteurization donor human milk Item: Title: 3.6 Bacillus cereus sensu lato in pre- and post-pasteurization donor human milk Item: Title: 3.7 Global donor human milk discard rate Item: Title: 4 Conclusion Item: Title: Acknowledgments Item: Title: References Info: Title: Impact of microbiological testing protocols on discard rates in donor human milk with a focus on Bacillus cereus Author: Vanessa Pleguezuelos Subject: Pleguezuelos V, López N, Trujillo AJ, ​Hernández-Herrero MM and Soria G (2026) Impact of microbiological testing protocols on discard rates in donor human milk with a focus on Bacillus cereus. Front. 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Front. Microbiol. 17:1887353. doi: 10.3389/fmicb.2026.1887353 Bacillus cereus donor human milk Enterobacteriaceae human milk human milk bank microbiological testing pasteurization Staphylococcus aureus Copyright © 2026 Pleguezuelos, López, Trujillo, Hernández-Herrero and Soria. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Checksum: 8c52d038 Type: CRC32 Checksum: a2514a117a114cb4a71ba4984e43b184 Type: MD5 Checksum: 83e818a49ce8282044e57dea5ef98e73c1b3d9c3 Type: SHA-1 Checksum: 1a5866cc03dabeb747359554362da8c8f67ea58bb571d4cd3cbd820b3bd5b37c Type: SHA-256