bcb33a6cd0c08875c6f5a16702892286 26TV37_SciAdv_Valls_SColl_pub_compressed.pdf dafd99d39edc9813bc43daa0873602e038d96b86 26TV37_SciAdv_Valls_SColl_pub_compressed.pdf 0d5939d7f78443a36936e9d491b367e1df3494c2b9f6b91ddd5389fc9c9cf6d4 26TV37_SciAdv_Valls_SColl_pub_compressed.pdf Title: SRF3 couples cellular iron homeostasis and defense responses through ubiquitin-dependent nano-organization Subject: Sci. Adv. 2026.12:eaeh4671 Producer: iLovePDF CreationDate: Thu Oct 15 16:25:46 2026 CEST ModDate: Fri Oct 23 13:28:30 2026 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 23 Encrypted: no Page size: 594 x 756 pts Page rot: 0 File size: 565929 bytes Optimized: no PDF version: 1.4 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TMBOZB+MyriadPro-Regular Type 1C Custom yes yes yes 29 0 XACHDT+MyriadPro-It Type 1C Custom yes yes yes 34 0 WBNBPV+MyriadPro-Bold Type 1C Custom yes yes yes 39 0 UYOPNZ+MyriadPro-Light Type 1C WinAnsi yes yes yes 45 0 TUZJZB+MyriadPro-Semibold Type 1C Custom yes yes yes 49 0 SQPDLD+MinionPro-Regular Type 1C Custom yes yes yes 54 0 QNQRJH+MinionPro-It Type 1C Custom yes yes yes 59 0 OKMFHL+STIX-Regular Type 1C Custom yes yes yes 64 0 Times-Roman Type 1 WinAnsi no no no 71 0 OKMFHL+STIX-Bold Type 1C Custom yes yes yes 340 0 NLCZTN+MyriadPro-BoldIt Type 1C WinAnsi yes yes yes 389 0 NLCZTN+STIX-Italic Type 1C WinAnsi yes yes yes 483 0 Helvetica-Bold Type 1 WinAnsi no no no 775 0 Helvetica Type 1 WinAnsi no no no 776 0 Helvetica-Oblique Type 1 WinAnsi no no no 777 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2026-10-24 03:57:54 CEST RepresentationInformation: 26TV37_SciAdv_Valls_SColl_pub_compressed.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2026-10-23 15:50:45 CEST Size: 565929 Format: PDF Version: 1.4 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 828 FreeObjects: 9 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: SRF3 couples cellular iron homeostasis and defense responses through ubiquitin-dependent nano-organization Destination: 1 Children: Item: Title: INTRODUCTION Destination: 1 Item: Title: RESULTS Destination: 1 Children: Item: Title: ROS burst triggers a transient decrease of ferrous iron upon flg22 treatment Destination: 1 Item: Title: Flg22-induced ROS production mediates low ferrous iron levels and engages SRF3-dependent iron homeostasis regulation Destination: 1 Item: Title: Clathrin-dependent endocytosis controls SRF3 plasma membrane levels upon flg22-induced low ferrous iron Destination: 1 Item: Title: flg22-triggered SRF3 iron-dependent endocytosis is mediated by ubiquitination Destination: 1 Item: Title: SRF3 segregates into mobile and immobile fractions at the plasma membrane in a ubiquitin-dependent manner Destination: 1 Item: Title: SRF3 is endocytosed from immobile nanoclusters Destination: 1 Item: Title: Ubiquitination of five lysine residues in SRF3 is required for root responses to low iron but not to flg22 Destination: 1 Item: Title: Ubiquitination of five lysine residues in SRF3 fine-tunes intracellular Fe2+ levels upon flg22 perception to modulate immune responses Destination: 1 Item: Title: SRF3 and its ubiquitination shape plant-microbe interactions Destination: 1 Item: Title: DISCUSSION Destination: 1 Item: Title: MATERIALS AND METHODS Destination: 1 Children: Item: Title: Plant materials and growth conditions Destination: 1 Item: Title: Plant transformation and selection Destination: 1 Item: Title: Phenotyping of early root growth responses Destination: 1 Item: Title: Phenotyping of late root growth responses Destination: 1 Item: Title: Microscopy setup Destination: 1 Item: Title: FRAP Destination: 1 Item: Title: FRET-FLIM Destination: 1 Item: Title: TIRF microscopy Destination: 1 Item: Title: Spt-PALM acquisition Destination: 1 Item: Title: Western blot Destination: 1 Item: Title: Immunoprecipitation Destination: 1 Item: Title: Mass spectrometry Destination: 1 Item: Title: Iron treatments, flg20 and flg22 treatments Destination: 1 Item: Title: Chemical treatments with BFA, Wm, ES9–17, Dark, CHX and β-Estradiol Destination: 1 Item: Title: Dye treatment with RhoNox-1, and DHE staining Destination: 1 Item: Title: ROS burst measurement Destination: 1 Item: Title: SRF3 Transcript Expression by RT-qPCR Destination: 1 Item: Title: Iron concentration measurement Destination: 1 Item: Title: RNA-seq Destination: 1 Item: Title: Differentially expressed genes (DEGs) Destination: 1 Item: Title: ANOVA-filtered gene set for clustering Destination: 1 Item: Title: Clustering Destination: 1 Item: Title: Aniline blue staining Destination: 1 Item: Title: CHA0 bacterial strain growth conditions and inoculation Destination: 1 Item: Title: R. solanacearum growth conditions and pathogenicity assays Destination: 1 Item: Title: In vitro inoculation and bacterial colonization assays Destination: 1 Item: Title: Symptom recording assays Destination: 1 Item: Title: Quantification and statistics Destination: 1 Item: Title: Supplementary Materials Destination: 1 Children: Item: Title: The PDF file includes: Destination: 1 Item: Title: Other Supplementary Material for this manuscript includes the following: Destination: 1 Item: Title: REFERENCES Destination: 1 Item: Title: Acknowledgments Destination: 1 Info: Title: SRF3 couples cellular iron homeostasis and defense responses through ubiquitin-dependent nano-organization Subject: Sci. 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