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ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: English Outlines: Item: Title: Electrochemical biosensor for early Alzheimer’s detection and patient risk stratification using plasma exosomes Destination: mk:title Children: Item: Title: 1 Introduction Destination: mk:H1_1 Item: Title: 2 Materials and methods Destination: mk:H1_2 Children: Item: Title: 2.1 Instrumentation and chemical reagents Destination: mk:H2_1 Item: Title: 2.2 Cell culturing, exosome isolation and purification Destination: mk:H2_2 Item: Title: 2.3 Human samples collection and processing Destination: mk:H2_3 Item: Title: 2.4 Characterization of the exosomes derived from SH-SY5Y cell line and from plasma samples Destination: mk:H2_4 Item: Title: 2.5 Capture efficiency of brain-derived exosomes on anti-NLGN3 magnetic particles Destination: mk:H2_5 Item: Title: 2.6 Magneto-actuated immunoassays for brain-derived exosomes using novel biomarkers NLGN3 and BACE-1 Destination: mk:H2_6 Item: Title: 2.7 Electrochemical biosensor targeting brain-derived exosomes at different stages of AD Destination: mk:H2_7 Item: Title: 2.8 Statistical analysis Destination: mk:H2_8 Item: Title: 3 Results and discussion Destination: mk:H1_3 Children: Item: Title: 3.1 NTA and Cryo-TEM characterization of the exosomes derived from SH-SY5Y cell line and plasma samples Destination: mk:H2_9 Item: Title: 3.2 Flow cytometry characterization of the exosomes derived from SH-SY5Y cell line Destination: mk:H2_10 Item: Title: 3.3 Capture efficiency of brain-derived exosomes on anti-NLGN3 magnetic particles Destination: mk:H2_11 Item: Title: 3.4 Electrochemical biosensor for the detection of brain derived exosomes in plasma Destination: mk:H2_12 Item: Title: 4 Conclusions Destination: mk:H1_4 Item: Title: CRediT authorship contribution statement Destination: mk:H1_5 Item: Title: Declarations Destination: mk:H1_6 Item: Title: Declaration of competing 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Biosensors and Bioelectronics, 292 (2026) 118061. doi:10.1016/j.bios.2025.118061 Alzheimer's disease Brain-derived exosomes NLGN3 BACE-1 Electrochemical biosensor Liquid biopsy Electrochemical biosensor for early Alzheimer's detection and patient risk stratification using plasma exosomes Rosanna Rossi Amanda Cano Arnau Pallarès-Rusiñol Agustín Ruiz Merce Martí Maria Isabel Pividori VoR 6th October 2025 Acrobat Distiller 8.1.0 (Windows) Alzheimer's disease,Brain-derived exosomes,NLGN3,BACE-1,Electrochemical biosensor,Liquid biopsy 6th October 2025 10.1016/j.bios.2025.118061 noindex journal © 2025 The Authors. 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