In vitro studies have long been used to investigate cell-substrate interactions to understand the effects of materials on the biologic environment in which they are inserted. This kind of study is very useful for detailed analysis of cell behaviour in contact with biomaterials, especially titanium, which is the most frequently used metal for both dental and orthopedic endosseous implants. In particular, osteoblasts respond to different surface topographies altering cell attachment, morphology, proliferation, and differentiation.