Expression patterns of Gbdac during leg development are dynamic, although Dmdac is expressed as a single ring in the Drosophila leg imaginal disc throughout its development. Gbdac expression is first detected in an anterior patch of cells in the leg bud at stage 7. At stage 8, the Gbdac expression domain becomes a narrow circumferential ring in the middle of the leg bud. At stage 9, the expression domain divides into two rings. Gbdac expression is not observed in the presumptive intersegmental border between the femur segment and more distal segments. Between stages 10 and 11, the proximal ring of expression corresponds to the distal part of the femur segment, while the distal ring of expression covers a region from the distal tibia to the proximal tarsus segment. In sagittal sections of the metathoracic femur, Gbdac expression is detected in the invaginating apodeme, as well as in the epithelial tissue of the distal femur.