Once the amphibian reaches the appropriate level of anesthesia for the procedures planned, remove the animal from the anesthetic bath and rinse with fresh water, or remove the topical preparation by rinsing. The animal will remain anesthetized for 10-80 minutes, depending on the method and drug concentration used. Recovery from anesthesia (righting reflex returns and animal able to move normally) will take 30-90 minutes after the animal is rinsed with fresh water.