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Gene therapy Destination: 1 Children: Item: Title: 1.1. Historical context and status Destination: 1 Item: Title: 1.2. Viral Vectors Destination: 1 Item: Title: 2. Adeno-associated virus (AAV) Destination: 1 Children: Item: Title: 2.1. Genome & Structure Destination: 1 Children: Item: Title: 2.1.1. Adeno-associated (AAV) genome Destination: 1 Item: Title: 2.1.2. Adenovirus helper functions Destination: 1 Item: Title: 2.2. Adeno-associated virus life cycle Destination: 1 Item: Title: 2.3. Adeno-associdated virus as gene delivery vector Destination: 1 Item: Title: 3. Strategies for rAAV production Destination: 1 Children: Item: Title: 3.1. Transient Gene Expresion (TGE) Destination: 1 Children: Item: Title: 3.1.1. Batch cultures Destination: 1 Item: Title: 3.1.2. Extended Gene Expression Protocol (EGE) Destination: 1 Item: Title: 4. Metabolic Engineering to increase the rAAV production and egrees rate Destination: 1 Item: Title: Objectives Destination: 1 Item: Title: Materials and Methods Destination: 1 Children: Item: Title: Cell line and culture conditions Destination: 1 Item: Title: Toxicity Assays Destination: 1 Item: Title: Plasmids design for generation of the stable cell line, amplification and purification Destination: 1 Item: Title: Pool generation by Random Integration Destination: 1 Item: Title: Digital droplet (ddPCR) for Copy Number Variation (CNV) determination Destination: 1 Item: Title: rAAV9 production by Transient Transfection and EGE Destination: 1 Item: Title: Flow Cytometry Destination: 1 Item: Title: Quantitative PCR (qPCR) for rAAV9 quantification Destination: 1 Item: Title: Results Destination: 1 Children: Item: Title: Toxicity Assay Destination: 1 Item: Title: Pool Generation Destination: 1 Item: Title: ddPCR Destination: 1 Item: Title: rAAV9 Production in SNAP47 Pool and WT Cells Destination: 1 Item: Title: Discussion Destination: 1 Item: Title: Conclusions Destination: 1 Item: Title: Bibliography Destination: 1 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1069 ImageHeight: 561 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Interpolate: true Image: NisoImageMetadata: FormatName: image/png ImageWidth: 615 ImageHeight: 230 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Interpolate: false Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 1065 ImageHeight: 729 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Interpolate: true Image: NisoImageMetadata: FormatName: image/png ImageWidth: 864 ImageHeight: 728 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Interpolate: false Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: 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