09b5bcd8e2ce086b0f2e4338ec00d22e CoelloQuihuiriEdwin_TFM.pdf 8675a0c0a896f42cb74f81f9a9328312a3a6ec8f CoelloQuihuiriEdwin_TFM.pdf 2b21d6697bc2a181c92d4eaf0ad15f571beb57510d1a299a7c69a9dfb6a2c73b CoelloQuihuiriEdwin_TFM.pdf Producer: 3-Heights™ PDF Merge Split Shell 6.12.1.11 (http://www.pdf-tools.com) ModDate: Mon Sep 22 12:46:11 2025 CEST Custom Metadata: no Metadata Stream: no Tagged: yes UserProperties: no Suspects: no Form: none JavaScript: no Pages: 32 Encrypted: no Page size: 612 x 792 pts (letter) Page rot: 0 File size: 1185883 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BCDEEE+Calibri TrueType WinAnsi yes yes no 10 0 BCDFEE+Calibri CID TrueType Identity-H yes yes yes 14 0 BCDGEE+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 21 0 BCDHEE+TimesNewRomanPSMT TrueType WinAnsi yes yes no 29 0 BCDIEE+TimesNewRomanPS-BoldMT TrueType WinAnsi yes yes no 32 0 BCDJEE+TimesNewRomanPS-BoldMT CID TrueType Identity-H yes yes yes 36 0 BCDNEE+TimesNewRomanPS-ItalicMT CID TrueType Identity-H yes yes yes 616 0 BCDOEE+TimesNewRomanPS-ItalicMT TrueType WinAnsi yes yes no 624 0 BCDKEE+ArialMT TrueType WinAnsi yes yes no 627 0 BCDLEE+Calibri-Light CID TrueType Identity-H yes yes yes 631 0 BCDMEE+Calibri-Light TrueType WinAnsi yes yes no 640 0 BCDPEE+SymbolMT CID TrueType Identity-H yes yes yes 731 0 BCEAEE+Calibri-Italic TrueType WinAnsi yes yes no 826 0 BCEBEE+CambriaMath CID TrueType Identity-H yes yes yes 856 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-09-30 03:31:38 CEST RepresentationInformation: CoelloQuihuiriEdwin_TFM.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-09-29 16:51:00 CEST Size: 1185883 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Tagged PDF PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: 4986a1bc6f3f9fc9f1c8e3df2f73e9052b86f32ee3973e9f8dd5194005bd1549.pdf Destination: 1 Item: Title: 4986a1bc6f3f9fc9f1c8e3df2f73e9052b86f32ee3973e9f8dd5194005bd1549.pdf Destination: 1 Children: Item: Title: Index Destination: 1 Item: Title: Summary Destination: 1 Item: Title: Abbreviation List Destination: 1 Item: Title: 1. Introduction Destination: 1 Children: Item: Title: 1.1. Fabry disease Destination: 1 Item: Title: 1.2. GLA Destination: 1 Item: Title: 1.3. Treatment Destination: 1 Item: Title: 1.4. Nanomedicine Destination: 1 Item: Title: 1.5. Extracelular Vesicles (EVs) Destination: 1 Item: Title: 1.6. General Objective Destination: 1 Item: Title: 1.7. Specific Objectives Destination: 1 Item: Title: 2. Materials and Methods Destination: 1 Children: Item: Title: 2.1. Plasmids production Destination: 1 Item: Title: 2.2. Production of GLA-HIS and GLA-Cmyc Destination: 1 Item: Title: 2.3. Clone stable CHO Destination: 1 Item: Title: 2.4. Purification of GLA soluble Destination: 1 Item: Title: 2.5. Quantification Western Blot WB Destination: 1 Item: Title: 2.6. Purification de EVs BTF y TFF Destination: 1 Children: Item: Title: 2.6.1. Bentochtop filtration (BTF) Destination: 1 Item: Title: 2.7. Size Exclusion Chromatography (SEC) Destination: 1 Item: Title: 2.8. Characterization of EVs Destination: 1 Children: Item: Title: 2.8.1. WB Destination: 1 Item: Title: 2.8.2. Nanoparticle Tracking Analysis NTA Destination: 1 Item: Title: 2.8.3. Transmission Electron Microscopy Destination: 1 Item: Title: 2.8.4. Enzymatic Activity Destination: 1 Item: Title: 3. Results Destination: 1 Children: Item: Title: 3.1. Protein production Destination: 1 Item: Title: 3.2. Purification of EVs Destination: 1 Item: Title: 3.3. Discussion Destination: 1 Item: Title: 4. Conclusions Destination: 1 Item: Title: 5. 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