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Introduction Destination: 1 Children: Item: Title: 1.1. Inflammation Destination: 1 Item: Title: 1.2. Exosomes Destination: 1 Item: Title: 1.3. Acute pancreatitis Destination: 1 Item: Title: 2. Initial hypothesis and objectives Destination: 1 Item: Title: 3. Methodology Destination: 1 Children: Item: Title: 3.1. Cellular lines Destination: 1 Item: Title: 3.2. Exosome isolation and treatment Destination: 1 Item: Title: 3.3. Protein quantification by BCA Destination: 1 Item: Title: 3.4. Nanoparticle tracking analysis Destination: 1 Item: Title: 3.5. Western blot analysis Destination: 1 Item: Title: 3.6. Cells treatments Destination: 1 Item: Title: 3.7. RT-qPCR Destination: 1 Item: Title: 3.8. Exosomes and cells staining and cell uptake analysis Destination: 1 Item: Title: 3.9. Fluorescence microscopy and analysis Destination: 1 Item: Title: 3.10. Immunofluorescence analysis Destination: 1 Item: Title: 3.11. Statistical analysis Destination: 1 Item: Title: 4. Results Destination: 1 Children: Item: Title: 4.1. sEVs characterization Destination: 1 Item: Title: 4.2. Improvement of exosomes isolating process Destination: 1 Item: Title: 4.3. Effect of lactate on sEVs Destination: 1 Children: Item: Title: 4.3.1 Changes in macrophage gene expression induced by lactate-treated exosomes Destination: 1 Item: Title: 4.3.2 Influence of lactate concentration on exosomes Destination: 1 Item: Title: 4.3.3 Impact of lactate treatment duration on exosomes Destination: 1 Item: Title: 4.3.4 Potential STAT3 signal transduction pathway Destination: 1 Item: Title: 4.3.5 Exosomes uptake by macrophages Destination: 1 Item: Title: 5. Discussion and conclusions Destination: 1 Item: Title: 6. Bibliography Destination: 1 Item: Title: 7. 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