Survival analysis of F98 glioma rat cells following minibeam or broad-beam synchrotron radiation therapy
Gil, Silvia (Universitat Autònoma de Barcelona. Centre d'Estudis en Biofísica)
Sarun, Sukhéna (European Synchrotron Radiation Facility)
Biete Solá, Albert (Hospital Clínic i Provincial de Barcelona)
Prezado, Yolanda (European Synchrotron Radiation Facility)
Sabés i Xamaní, Manel (Universitat Autònoma de Barcelona. Centre d'Estudis en Biofísica)

Fecha: 2011
Resumen: Background: In the quest of a curative radiotherapy treatment for gliomas new delivery modes are being explored. At the Biomedical Beamline of the European Synchrotron Radiation Facility (ESRF), a new spatially-fractionated technique, called Minibeam Radiation Therapy (MBRT) is under development. The aim of this work is to compare the effectiveness of MBRT and broad-beam (BB) synchrotron radiation to treat F98 glioma rat cells. A dose escalation study was performed in order to delimit the range of doses where a therapeutic effect could be expected. These results will help in the design and optimization of the forthcoming in vivo studies at the ESRF. Methods: Two hundred thousand F98 cells were seeded per well in 24-well plates, and incubated for 48 hours before being irradiated with spatially fractionated and seamless synchrotron x-rays at several doses. The percentage of each cell population (alive, early apoptotic and dead cells, where either late apoptotic as necrotic cells are included) was assessed by flow cytometry 48 hours after irradiation, whereas the metabolic activity of surviving cells was analyzed on days 3, 4, and 9 post-irradiation by using QBlue test. Results:.
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Lengua: Anglès.
Documento: article ; publishedVersion
Publicado en: Radiation oncology, Vol. 6, N. 37 (April 2011) , p. 1-9, ISSN 1748-717X

PMID: 21489271

9 p, 854.1 KB

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