The presence of a high-Km hexokinase activity in dog, but not in boar, sperm
Fernández Novell, Josep M.. (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Ballester i Casals, Joan (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Medrano, Antonio (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Otaegui Goya, Pedro José (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Rigau i Mas, Teresa (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Guinovart Cirera, Joan J (Universitat de Barcelona. Departament de Bioquímica i Biologia Molecular)
Rodríguez Gil, Joan Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Data:	2004
Resum:	The presence of a high-Km hexokinase activity was tested in both dog and boar spermatozoa. Hexokinase kinetics from dog extracts showed the presence of a specific activity (dog-sperm glucokinase-like protein, DSGLP), in the range of glucose concentrations of 4-10 mM, whereas boar sperm did not show any DSGLP activity. Furthermore, dog-sperm cells, but not those of boar, showed the presence of a protein which specifically reacted against a rat-liver anti-glucokinase antibody. This protein also had a molecular weight equal to that observed in rat-liver extracts, suggesting a close similarity between both the proteins. This glucokinase-like protein was distributed in the peri- and post-acrosomal zones of the head, and the midpiece and principal piece of tail of dog spermatozoa. These results indicate that dog spermatozoa have functional high-Km hexokinase activity, which could contribute to a very fine regulation of their hexose metabolism. This strict regulation could ultimately be very important in optimizing dog-sperm function along its life-time.
Drets:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	High-Km hexokinase ; Dog-sperm ; Glucokinase-regulatory
Publicat a:	FEBS letters, Vol. 570, Num. 1-3 (2004) , p. 211-216, ISSN 1873-3468

DOI: 10.1016/j.febslet.2004.06.015

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