Biodiesel synthesis in a solvent-free system by recombinant Rhizopus oryzae Lipase : study of the catalytic reaction progress
Canet Morral, Albert (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Benaiges, M. Dolors (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Valero Barranco, Francisco (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)

Data:	2014
Resum:	The recombinant 1,3-positional selective Rhizopus oryzae lipase (rROL) was used to synthesize biodiesel and monoacylglycerols simultaneously. The reaction was carried out in a solvent-free system with the enzyme immobilized on octadecyl-Sepabeads. Using response surface methodology, the methyl ester yield was optimized by means of the study of the effect of water, substrate molar ratio (methanol:olive oil) and methanol stepwise addition. It was concluded that in order to prevent enzyme inactivation by methanol, alcohol should be added slowly; otherwise a large amount of water would be present. Taking the best conditions, a 50. 3 % yield was achieved in 3 h, which corresponds to 75. 4 % of the acyl groups at the 1,3-position undergoing transesterification. It was also concluded that methyl esters result from the esterification of the free fatty acid hydrolyzed by the enzyme and also from a direct transesterification of oil. In addition, the fatty acid selectivity of rROL was found not to favor one fatty acid in olive oil over another.
Ajuts:	Ministerio de Ciencia e Innovación CTQ2010-15131 Generalitat de Catalunya 2009/SGR-281 Generalitat de Catalunya 2010/CONE3-00063
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Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Matèries del catàleg ; Immobilization ; Biodiesel ; Monoacylglycerols ; Recombinant Rhizopus oryzae lipase ; Response surface model
Publicat a:	Journal of the American Oil Chemists' Society, Vol. 91, Num. 9 (2014) , p. 1499-1506, ISSN 0003-021X

DOI: 10.1007/s11746-014-2498-y

Postprint 26 p, 741.0 KB

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