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Pàgina inicial > Articles > Articles publicats > Study of protein expresion in peri-infarct tissue after cerebral ischemia |
Data: | 2015 |
Resum: | In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contra-lateral tissues (at least 3-fold, p < 0. 05). Using COFRADIC, 5412 proteins were identified, with 72 showing a difference in expression. Apart from blood-related proteins (such as serum albumin), both techniques showed that the 70 kDa family of heat shock proteins were highly expressed in the peri-infarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealed that only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressed by the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c) is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarct tissue in the ischemic brain. |
Ajuts: | Instituto de Salud Carlos III PI11/02161 Instituto de Salud Carlos III CP09/00074 Instituto de Salud Carlos III RETICS/R012/0014 Ministerio de Economía y Competitividad SAF2008-02190 |
Nota: | Altres ajuts: programa Miguel Servet program i programa Sara Borrell |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Proteòmica ; Isquèmia cerebral ; Proteomic analysis ; Stroke |
Publicat a: | Scientific reports, Vol. 5 (2015) , article 12030, ISSN 2045-2322 |
11 p, 1.4 MB |