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A highly versatile and easily configurable system for plant electrophysiology
Gunsé Forcadell, Benito (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Poschenrieder Wiens, Charlotte (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)
Rankl, Simone (Helmholtz Zentrum München, Germany)
Schröeder, Peter (Helmholtz Zentrum München, Germany)
Rodrigo Moreno, Ana (International Laboratory of Plant Neurobiology (LINV), Italy)
Barceló Coll, Juan (Universitat Autònoma de Barcelona. Departament de Biologia Animal, de Biologia Vegetal i d'Ecologia)

Data: 2016
Resum: In this study we present a highly versatile and easily configurable system for measuring plant electrophysiological parameters and ionic flow rates, connected to a computer-controlled highly accurate positioning device. The modular software used allows easy customizable configurations for the measurement of electrophysiological parameters. Both the operational tests and the experiments already performed have been fully successful and rendered a low noise and highly stable signal. Assembly, programming and configuration examples are discussed. The system is a powerful technique that not only gives precise measuring of plant electrophysiological status, but also allows easy development of ad hoc configurations that are not constrained to plant studies. 1)We developed a highly modular system for electrophysiology measurements that can be used either in organs or cells and performs either steady or dynamic intra- and extracellular measurements that takes advantage of the easiness of visual object-oriented programming. 2)High precision accuracy in data acquisition under electrical noisy environments that allows it to run even in a laboratory close to electrical equipment that produce electrical noise. 3)The system makes an improvement of the currently used systems for monitoring and controlling high precision measurements and micromanipulation systems providing an open and customizable environment for multiple experimental needs.
Nota: Número d'acord de subvenció MINECO/BFU2013/42839-R
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: article ; recerca ; publishedVersion
Matèria: Plant root ; Membrane potential ; Ion flux measurement ; Electrophysiology
Publicat a: MethodsX, Vol. 3 (2016) , p. 436-451, ISSN 2215-0161

DOI: 10.1016/j.mex.2016.05.007
PMID: 27298766

16 p, 2.7 MB

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