Long-term exercise modulates hippocampal gene expression in sencescent females mice
Álvarez Torres, María Jesús (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Castro-Freire, Marco (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Cosin-Tomas, Marta (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Sanchez-Roige, Sandra (Universitat Autònoma de Barcelona. Institut de Neurociències)
F. Lalanza, Jaume (Universitat Autònoma de Barcelona. Institut de Neurociències)
Del Valle, Jaume (Universitat de Barcelona. Institut de Biomedicina)
Párrizas, Marcelina (Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas)
Camins, Antoni (Universitat de Barcelona. Institut de Biomedicina)
Pallàs, Mercè (Universitat de Barcelona. Institut de Biomedicina)
Escorihuela, Rosa M. (Universitat Autònoma de Barcelona. Institut de Neurociències)
Kaliman, Perla (Institut d'Investigacions Biomèdiques August Pi i Sunyer)

Data:	2013
Resum:	The senescence-accelerated SAMP8 mouse is considered a useful non-transgenic model for studying aspects of progressive cognitive decline and Alzheimer's disease (AD). Using SAMR1 mice as controls, here we explored the effects of 6 months of voluntary wheel running in 10-month-old female SAMP8 mice. Exercise in SAMP8 mice improved phenotypic features associated with premature aging (i. e. , skin color and body tremor) and enhanced vascularization and BDNF gene expression in the hippocampus compared with controls. With the aim of identifying genes involved in brain aging responsive to long-term exercise, we performed whole genome microarray studies in hippocampus from sedentary SAMP8 (P8sed), SAMR1 (R1sed), and exercised SAMP8 (P8run) mice. The genes differentially expressed in P8sed versus R1sed were considered as putative aging markers (i) and those differentially expressed in P8run versus P8sed were considered as genes modulated by exercise (ii). Genes differentially expressed in both comparisons (i and ii) were considered as putative aging genes responsive to physical exercise. We identified 34 genes which met both criteria. Gene ontology analysis revealed that they are mainly involved in functions related to extracellular matrix maintenance. Selected genes were validated by real-time quantitative PCR assays, i. e. , collagen type 1 alpha 1 (col1a1), collagen type 1 alpha 2 (col1a2), fibromodulin (fmod), prostaglandin D(2) synthase (ptgds), and aldehyde dehydrogenase (Aldh1a2). As a whole, our study suggests that exercise training during adulthood may prevent or delay gene expression alterations and processes associated with hippocampal aging in at-risk subjects.
Ajuts:	Ministerio de Ciencia e Innovación SAF2010-15050 Instituto de Salud Carlos III DPS2008-06998-C01 Instituto de Salud Carlos III DPS2008-06998-C02 Instituto de Salud Carlos III PI080400 Ministerio de Ciencia e Innovación BFU2009-09988/BMC Ministerio de Ciencia e Innovación SAF2009-13093 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-00853
Nota:	Altres ajuts: FI-DGR 2011 de la Generalitat de Catalunya
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Aging ; Alzheimer's disease ; Brain ; Exercise ; Gene ; Long-term ; Mice ; Microarrays ; SAMP8 ; Voluntary
Publicat a:	Journal of Alzheimer's disease, Vol. 33, Num. 4 (2013) , p. 1177-1190, ISSN 1875-8908

DOI: 10.3233/JAD-121264

14 p, 335.5 KB

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