Dose-dependent differential effect of neurotrophic factors on in vitro and in iivo regeneration of motor and sensory neurons
Santos, Daniel (Universitat Autònoma de Barcelona. Institut de Neurociències)
González Pérez, Francisco J. (Universitat Autònoma de Barcelona. Institut de Neurociències)
Navarro, X. (Xavier) (Xavier) (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Del Valle, Jaume (Universitat Autònoma de Barcelona. Institut de Neurociències)

Data:	2016
Resum:	Although peripheral axons can regenerate after nerve transection and repair, functional recovery is usually poor due to inaccurate reinnervation. Neurotrophic factors promote directional guidance to regenerating axons and their selective application may help to improve functional recovery. Hence, we have characterized in organotypic cultures of spinal cord and dorsal root ganglia the effect of GDNF, FGF-2, NGF, NT-3, and BDNF at different concentrations on motor and sensory neurite outgrowth. In vitro results show that GDNF and FGF-2 enhanced both motor and sensory neurite outgrowth, NGF and NT-3 were the most selective to enhance sensory neurite outgrowth, and high doses of BDNF selectively enhanced motor neurite outgrowth. Then, NGF, NT-3, and BDNF (as the most selective factors) were delivered in a collagen matrix within a silicone tube to repair the severed sciatic nerve of rats. Quantification of Fluorogold retrolabeled neurons showed that NGF and NT-3 did not show preferential effect on sensory regeneration whereas BDNF preferentially promoted motor axons regeneration. Therefore, the selective effects of NGF and NT-3 shown in vitro are lost when they are applied in vivo, but a high dose of BDNF is able to selectively enhance motor neuron regeneration both in vitro and in vivo.
Ajuts:	European Commission 280778 European Commission 611687 Instituto de Salud Carlos III CIBERNED/CB06/05/1105 Instituto de Salud Carlos III TERCEL/RD12/0019/001
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Neural plasticity, Vol. 2016 (September 2016) , Article ID 4969523, ISSN 1687-5443

Adreça alternativa: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5102746/
Adreça alternativa: https://www.hindawi.com/journals/np/2016/4969523/
DOI: 10.1155/2016/4969523
PMID: 27867665

14 p, 5.6 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Institut de Neurociències (INc)
Articles > Articles de recerca
Articles > Articles publicats