Mutations related to Antiretroviral Resistance identified by ultra-deep sequencing in HIV-1 infected children under Structured Interruptions of HAART
Vázquez Guillén, José Manuel (Universidad Autónoma de Nuevo León (Mèxic))
Palacios Saucedo, Gerardo C. (Instituto Mexicano del Seguro Social)
Rivera Morales, Lydia G. (Universidad Autónoma de Nuevo León (Mèxic))
García Campos, Jorge (Instituto Mexicano del Seguro Social)
Ortiz López, Rocío (Universidad Autónoma de Nuevo León (Mèxic))
Noguera-Julian, Marc (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Paredes, Roger (Institut Germans Trias i Pujol. Institut de Recerca de la Sida IrsiCaixa)
Vielma Ramírez, Herlinda J. (Universidad Autónoma de Nuevo León (Mèxic))
Ramírez, Teresa (Laboratorio Estatal de Salud Pública del Estado de Nuevo León (Mèxic))
Chávez García, Marcelino (Instituto Mexicano del Seguro Social)
López Guillén, Paulo (Instituto Mexicano del Seguro Social)
Briones Lara, Evangelina (Instituto Mexicano del Seguro Social)
Sánchez Sánchez, Luz M. (Instituto Mexicano del Seguro Social)
Vázquez Martínez, Carlos A. (Instituto Mexicano del Seguro Social)
Rodríguez Padilla, Cristina (Universidad Autónoma de Nuevo León (Mèxic))

Data:	2016
Resum:	Although Structured Treatment Interruptions (STI) are currently not considered an alternative strategy for antiretroviral treatment, their true benefits and limitations have not been fully established. Some studies suggest the possibility of improving the quality of life of patients with this strategy; however, the information that has been obtained corresponds mostly to studies conducted in adults, with a lack of knowledge about its impact on children. Furthermore, mutations associated with antiretroviral resistance could be selected due to sub-therapeutic levels of HAART at each interruption period. Genotyping methods to determine the resistance profiles of the infecting viruses have become increasingly important for the management of patients under STI, thus low-abundance antiretroviral drug-resistant mutations (DRM's) at levels under limit of detection of conventional genotyping (<20% of quasispecies) could increase the risk of virologic failure. In this work, we analyzed the protease and reverse transcriptase regions of the pol gene by ultra-deep sequencing in pediatric patients under STI with the aim of determining the presence of high- and low-abundance DRM's in the viral rebounds generated by the STI. High-abundance mutations in protease and high- and low-abundance mutations in reverse transcriptase were detected but no one of these are directly associated with resistance to antiretroviral drugs. The results could suggest that the evaluated STI program is virologically safe, but strict and carefully planned studies, with greater numbers of patients and interruption/restart cycles, are still needed to evaluate the selection of DRM's during STI.
Nota:	Altres ajuts: CONACYT/GCPS/44519
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Sida ; Tractament ; VIH (Virus) ; Antiretroviral resistance ; Structured interruptions of HAART ; Structured Treatment Interruptions (STI)
Publicat a:	PloS one, Vol. 11 Núm. 1 (January 2016) , ISSN 1932-6203

DOI: 10.1371/journal.pone.0147591
PMID: 26807922

11 p, 203.3 KB

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