Web of Science: 18 cites, Scopus: 16 cites, Google Scholar: cites
Genome-wide analysis of polycistronic microRNAs in cultivated and wild rice
Baldrich, Patricia (Centre de Recerca en Agrigenòmica)
Hsing, Yue-Ie Caroline (Academia Sinica (Taiwan). Institute of Plant and Microbial Biology)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Data: 2016
Resum: MicroRNAs (miRNAs) are small noncoding RNAs that direct posttranscriptional gene silencing in eukaryotes. They are frequently clustered in the genomes of animals and can be independently transcribed or simultaneously transcribed into single polycistronic transcripts. Only a few miRNA clusters have been described in plants, and most of them are generated from independent transcriptional units. Here, we used a combination of bioinformatic tools and experimental analyses to discover new polycistronic miRNAs in rice. A genome-wide analysis of clustering patterns of MIRNA loci in the rice genome was carried out using a criterion of 3 kb as the maximal distance between two miRNAs. This analysis revealed 28 loci with the ability to form the typical hairpin structure of miRNA precursors in which 2 or more mature miRNAs mapped along the same structure. RT-PCR provided evidence for the polycistronic nature of seven miRNA precursors containing homologous or nonhomologous miRNA species. Polycistronic miRNAs and candidate polycistronic miRNAs are located across different rice chromosomes, except chromosome 12, and resided in both duplicated and nonduplicated chromosomal regions. Finally, most polycistronic and candidate polycistronic miRNAs showed a pattern of conservation in the genome of rice species with an AA genome. The diversity in the organization of MIR genes that are transcribed as polycistrons suggests a versatile mechanism for the control of gene expression in different biological processes and supports additional levels of complexity in miRNA functioning in plants.
Ajuts: Ministerio de Economía y Competitividad BIO2015-67212-R
Ministerio de Economía y Competitividad BIO2012-32838
Ministerio de Ciencia e Innovación BES-2010-032879
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Genome duplication ; Oryza sativa ; Polycistronic miRNAs ; Target gene ; Wild rice
Publicat a: Genome biology and evolution, Vol. 8, no. 4 (April 2016) , p. 1104-114, ISSN 1759-6653

DOI: 10.1093/gbe/evw062
PMID: 27190137


11 p, 538.9 KB

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