Web of Science: 6 cites, Scopus: 6 cites, Google Scholar: cites,
Persistence of respiratory and inflammatory responses after dermal sensitization to persulfate salts in a mouse model of non-atopic asthma
Cruz Carmona, María Jesús (Hospital Universitari Vall d'Hebron)
Ollé Monge, Marta (Universitat Autònoma de Barcelona. Departament de Medicina)
Vanoirbeek, J. A. (Centre of Environment and Health, KU Leuven)
Assialioui, A. (Hospital Universitari Vall d'Hebron)
Gómez Ollés, Susana (Hospital Universitari Vall d'Hebron)
Muñoz, Xavier (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data: 2016
Resum: Exposure to ammonium persulfate (AP) has been reported to be the main cause of occupational asthma in hairdressers. The aim of this study is to assess how long the asthmatic response to AP can be induced after dermal sensitization in a mouse model. BALB/c mice received dermal applications of AP or dimethylsulfoxide (DMSO) (control) on days 1 and 8. They then received a single nasal instillation (challenge) of AP or saline on days 15, 22, 29, 36, 45, 60 and 90. Respiratory responsiveness to methacholine was measured 24 h after the challenge using a non-specific methacholine provocation test. Pulmonary inflammation was analysed in bronchoalveolar lavage (BAL), and total serum immunoglobulin (Ig) E, IgG1 and IgG2a were measured in serum samples. Histological analysis of lung slides was performed. Mice dermally sensitized and intranasally challenged with AP showed respiratory responsiveness to methacholine as long as 45 days after initial sensitization, as well as increased percentage of neutrophils in BAL compared with the control group. At day 60, dermally sensitized mice still presented bronchial hyperresponsiveness, while the percentage of neutrophils returned to baseline levels similar to those of controls. Total serum IgE increased significantly on day 22 after dermal sensitization. Total serum IgG1 and IgG2a increased from 45 days after dermal sensitization and remained high at 90 days. Both respiratory responsiveness to methacholine and airway inflammation responses decrease with increasing time between sensitization and challenge. Respiratory responsiveness to methacholine tends to persist longer than inflammation.
Ajuts: Instituto de Salud Carlos III CP12/0310100229
FUCAP/FIS/PI10-00782
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès
Document: Article ; recerca ; Versió publicada
Matèria: Occupational asthma ; Airway hyperresponsiveness ; Lung inflammation ; Ammonium persulfate
Publicat a: Allergy, asthma, and clinical immunology, Vol. 12 (May 2016) , art. 26, ISSN 1710-1492

DOI: 10.1186/s13223-016-0131-3
PMID: 27222656


7 p, 1.1 MB

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