Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius
Cabañes Sáenz, Francisco Javier (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Castellà Gómez, Gemma (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Bragulat, M. Rosa (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Sanseverino, Walter (Centre de Recerca en Agrigenòmica)
Sánchez, Armand (Universitat Autònoma de Barcelona. Servei Veterinari de Genètica Molecular)
Aiese-Cigliano, Riccardo (Sequentia Biotech SL)

Date: 2015
Abstract: In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Micologia Veterinària ; Gene regulatory networks ; Fungal genomics
Published in: Scientific Reports, Març 2015, p. 1-7



7 p, 976.0 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Health sciences and biosciences > Veterinary Mycology Group
Articles > Research articles
Articles > Published articles

 Record created 2018-06-15, last modified 2019-09-17



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