Web of Science: 28 cites, Scopus: 29 cites, Google Scholar: cites
Potential of the solid-state fermentation of soy fibre residues by native microbial populations for bench-scale alkaline protease production
Abraham, Juliana (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gea Leiva, Teresa (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)
Sanchez, Antoni, dir. (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química)

Data: 2013
Resum: The production of alkaline proteases by solid-state fermentation (SSF) was evaluated. The effect of three agro-industrial residues was examined: coffee husk, hair waste from the tanning industry and soy fibre residues. Soy fibre presented the highest yield for protease production at the laboratory scale (37 °C, 100 g samples). Consequently, experiments with soy fibre (F) and soy fibre with 10% compost as an inoculum (FC) were performed for 14 days in 4. 5 L bench-scale aerobic near-adiabatic reactors. The highest activity occurred under thermophilic conditions and a high respiration activity of 47,331 ± 1391 U/g dry matter for F and 18,750 ± 1596 U/g dry matter for FC, which are much higher values than those reported in other studies. Alkaline proteases showed maximum stability at pH 11 and temperatures of 43. 8 °C (F) and 30 °C (FC), which was determined by a full factorial experimental design. Storage assays demonstrated that 90% of enzyme activity was preserved for three months by lyophilising or freezing the samples at −80 °C.
Ajuts: MICINN/CTM2012-33663
Drets: Tots els drets reservats
Llengua: Anglès
Document: article ; recerca ; Versió sotmesa a revisió
Matèria: Solid-state fermentation ; Alkaline protease ; Respiration activity ; Experimental design ; Bench scale production
Publicat a: Biochemical engineering journal, Vol. 74 (May 2013) , p. 15-19, ISSN 1369-703X

DOI: 10.1016/j.bej.2013.02.008


Preprint
18 p, 419.6 KB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Enginyeries > Grup de Recerca en Compostatge (GICOM)
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