Web of Science: 2 cites, Scopus: 2 cites, Google Scholar: cites,
Glutamic acid independent production of bioflocculants by bacillus subtilis UPMB13
Zulkeflee, Zufarzaana (University Putra Malaysia. Department of Environmental Sciences)
Shamsuddin, Zulkifli (University Putra Malaysia. Department of Land Management)
Aris, Ahmad Zaharin (University Putra Malaysia. Environmental Forensics Research Centre)
Yusoff, Mohd Kamil (University Putra Malaysia. UPM Consultancy and Services Sdn. Bhd.)
Komilis, Dimitrios (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sánchez Ferrer, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data: 2016
Resum: Bacillus subtilis UPMB13 was found to be an L-glutamic acid independent producer of extracellular polymeric substances (EPS) with bioflocculation properties. Optimum production of the bioflocculant was found to be at the early stage of cell propagation of 24-72 h of fermentation. At a limited nutrient input of 100 mL tryptic soy broth, the flocculating activities were found to be negatively correlated (p < 0. 01) with growth as it continued to decline after 72 h, while cell growth proliferated further. Ample nutrient supply may prolong bioflocculant production with flocculating activities of 90 % and higher, while excess oxygen supply may promote rapid growth that can lead to poor flocculation due to the re-use of the bioflocculant as a substitute for food during starvation. Bioflocculant production occurred at best at 25-30 °C incubation temperature and at the initial pH medium of 7 to 8. The bioflocculant was proven to be extracellularly produced as the broth and the supernatant possessed the ability to flocculate the suspended kaolin particles. Bioflocculant productions by UPMB13 were hereditarily stable among succeeding progenies, hence, proving genetic competency. About 0. 90 g of purified bioflocculant were collected from 1 L culture broth of UPMB13 under the optimized fermentation conditions.
Ajuts: Ministerio de Economía y Competitividad CTM2015-69513-R
Drets: Tots els drets reservats
Llengua: Anglès
Document: Article ; recerca ; Versió sotmesa a revisió
Matèria: Extracellular polymeric substance (EPS) ; Biopolymer ; γ-PGA ; De-novo ; Kaolin assay
Publicat a: Environmental processes, Vol. 3, issue 2 (June 2016) , p. 353-367, ISSN 2198-7491

DOI: 10.1007/s40710-016-0161-3


Preprint
20 p, 261.2 KB

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Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Enginyeries > Grup de Recerca en Compostatge (GICOM)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2018-10-04, darrera modificació el 2021-09-26



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