Lgr5 Does Not Vary Throughout the Menstrual Cycle in Endometriotic Human Eutopic Endometrium
Vallvé Juanico, Júlia (Hospital Universitari Vall d'Hebron)
Barón, Cristian (Hospital Universitari Vall d'Hebron)
Suárez Salvador, Elena (Hospital Universitari Vall d'Hebron)
Castellvi, Josep (Hospital Universitari Vall d'Hebron)
Ballesteros, Agustín (Hospital Universitari Vall d'Hebron)
Gil-Moreno, Antonio 1965- (Universitat Autònoma de Barcelona)
Santamaria, Xavier (Igenomix Foundation)

Data:	2019
Resum:	Endometriosis is characterized by the abnormal presence of endometrium outside of the uterus, resulting in pelvic pain and infertility. The leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) has been postulated to be a marker of stem cells in the endometrium. However, LGR5 + cells have a macrophage-like phenotype in this tissue, so it is unclear what role LGR5 + cells actually play in the endometrium. Macrophages serve an important function in the endometrium to maintain fertility, while LGR5 + cells generally have a role in tumor progression and are involved in invasion in some cancers. We sought to determine whether LGR5 + cells vary across the menstrual cycle in women with endometriosis and whether there are implications for LGR5 in the aggressiveness of endometriosis and reproductive outcomes. We performed immunofluorescence, flow cytometry, and primary culture in vitro experiments on eutopic and ectopic endometrium from healthy and endometriosis patients and observed that neither LGR5 + cells nor LGR5 expression varied throughout the cycle. Interestingly, we observed that LGR5 + cell percentage overexpressing CD163 (anti-inflammatory marker) was higher in healthy endometrium, suggesting that in endometriosis, endometrium presents a more pro-inflammatory phenotype that likely leads to poor obstetric outcomes. We also observed higher levels of LGR5 + cells in ectopic lesions compared to eutopic endometrium and specifically in deep infiltrating endometriosis, indicating that LGR5 could be involved in progression and aggressiveness of the disease.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2015-SGR-074
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	LGR5 ; Endometrium ; Endometriosis ; Menstrual cycle ; Macrophages
Publicat a:	International journal of molecular sciences, Vol. 20, Núm. 1 (January 2019) , art. 22, ISSN 1422-0067

DOI: 10.3390/ijms20010022
PMID: 30577586

12 p, 2.5 MB

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