Web of Science: 36 cites, Scopus: 38 cites, Google Scholar: cites,
Fully printed one-step biosensing device using graphene/AuNPs composite
Nagar, Bhawna (Institut Català de Nanociència i Nanotecnologia)
Balsells, Marc (Institut Català de Nanociència i Nanotecnologia)
De La Escosura-Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Gómez-Romero, Pedro 1959- (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data: 2019
Resum: Driven by the growing need of simple, cost efficient and flexible sensing systems, we have designed here a fully printed Reduced Graphene Oxide (rGO) based impedimetric sensor for one step sensing of DNA. The DNA sensor was fabricated by stamping of layered rGO and rGO/gold nanoparticles/single stranded DNA (rGO/AuNPs/ssDNA) composites over PET substrates using wax-printing technique. rGO works as an excellent working electrode, while the AuNPs create a suitable environment for ssDNA immobilization. Counter and reference electrodes were previously screen-printed on the plastic substrate, making thus a compact and highly integrated sensing platform. The change in electron transfer resistance after hybridization with a target ssDNA specific of Coxsackie B3 virus was monitored using electrochemical impedance spectroscopy (EIS), finding a linear response in the range of concentrations 0. 01-20 µM. The novel, simple and straightforward one-step printing process for fabrication of a biosensing device developed keeps in mind the growing need of large scale device manufacturing. The successful proof-of-concept for the detection of DNA hybridization can be extended to other affinity biosensors, taking advantage of the integration of the bioreceptor on the sensor surface. Such ready-to-use biosensor would lead to a one-step electrochemical detection.
Ajuts: Ministerio de Economía y Competitividad MAT2017-87202-P
Ministerio de Economía y Competitividad SEV-2013-0295
European Commission 785219
Drets: Tots els drets reservats.
Llengua: Anglès
Document: Article ; recerca ; Versió sotmesa a revisió
Matèria: RGO ; Wax stamping ; Screen printing ; Electrochemistry ; DNA ; Biosensing
Publicat a: Biosensors and bioelectronics, Vol. 129 (March 2019) , p. 238-244, ISSN 1873-4235

DOI: 10.1016/j.bios.2018.09.073


Preprint
24 p, 1.1 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències > Institut Català de Nanociència i Nanotecnologia (ICN2)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2019-07-02, darrera modificació el 2023-10-01



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