A label-free nanostructured plasmonic biosensor based on Blu-ray discs with integrated microfluidics for sensitive biodetection
López Muñoz, Gerardo Arturo (Institut Català de Nanociència i Nanotecnologia)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Peláez Gutiérrez, Enelia Cristina (Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina)
Homs Corbera, Antoni (Institut Català de Nanociència i Nanotecnologia)
García-Hernandez, M. C. (Protein Alternatives. S.L.)
Imbaud, J. I. (Protein Alternatives. S.L.)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Data:	2017
Resum:	Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have developed a simple integrated nanoplasmonic sensor taking advantage of the periodic nanostructured array of commercial Blu-ray discs. Sensors with two gold film thicknesses (50 and 100 nm) were fabricated and optically characterized by varying the oblique-angle of the incident light in optical reflectance measurements. Contrary to the use normal light incidence previously reported with other optical discs, we observed an enhancement in sensitivity and a narrowing of the resonant linewidths as the light incidence angle was increased, which could be related to the generation of Fano resonant modes. The new sensors achieve a figure of merit (FOM) up to 35 RIU and a competitive bulk limit of detection (LOD) of 6. 3×10 RIU. These values significantly improve previously reported results obtained with normal light incidence reflectance measurements using similar structures. The sensor has been combined with versatile, simple, ease to-fabricate microfluidics. The integrated chip is only 1 cm (including a PDMS flow cell with a 50 µm height microfluidic channel fabricated with double-sided adhesive tape) and all the optical components are mounted on a 10 cm×10 cm portable prototype, illustrating its facile miniaturization, integration and potential portability. Finally, to assess the label-free biosensing capability of the new sensor, we have evaluated the presence of specific antibodies against the GTF2b protein, a tumor-associate antigen (TAA) related to colorectal cancer. We have achieved a LOD in the pM order and have assessed the feasibility of directly measuring biological samples such as human serum.
Ajuts:	Ministerio de Economía y Competitividad TEC2016-78515-R Ministerio de Economía y Competitividad RTC-2014-1518-1 Ministerio de Economía y Competitividad SEV-2013-0295 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624
Nota:	Altres ajuts: CONACYT, Grant/Award Number: 225362
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Matèria:	Blu-ray disc ; Plasmonic nanostructure ; Integration ; Microfluidics ; Biosensing ; Antibody
Publicat a:	Biosensors & bioelectronics, Vol. 96 (Oct. 2017) , p. 260-267, ISSN 1873-4235

DOI: 10.1016/j.bios.2017.05.020

Preprint 27 p, 548.5 KB

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