A RNA-seq analysis to describe the boar sperm transcriptome and its seasonal changes
Gòdia, Marta (Centre de Recerca en Agrigenòmica)
Estill, Molly (Wayne State University. Department of Obstetrics and Gynecology)
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica)
Balasch, Sam (Grup Gepork S.A.)
Rodríguez-Gil, Joan E. (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Krawetz, Stephen A. (Wayne State University. Department of Obstetrics and Gynecology)
Sánchez Bonastre, Armando (Centre de Recerca en Agrigenòmica)
Clop, Alex (Centre de Recerca en Agrigenòmica)
Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments

Date:	2019
Abstract:	Understanding the molecular basis of cell function and ultimate phenotypes is crucial for the development of biological markers. With this aim, several RNA-seq studies have been devoted to the characterization of the transcriptome of ejaculated spermatozoa in relation to sperm quality and fertility. Semen quality follows a seasonal pattern and decays in the summer months in several animal species. The aim of this study was to deeply profile the transcriptome of the boar sperm and to evaluate its seasonal changes. We sequenced the total and the short fractions of the sperm RNA from 10 Pietrain boars, 5 collected in summer and 5 five sampled in winter, and identified a complex and rich transcriptome with 4,436 coding genes of moderate to high abundance. Transcript fragmentation was high but less obvious in genes related to spermatogenesis, chromatin compaction and fertility. Short non-coding RNAs mostly included piwi-interacting RNAs, transfer RNAs and microRNAs. We also compared the transcriptome of the summer and the winter ejaculates and identified 34 coding genes and 7 microRNAs with a significantly distinct distribution. These genes were mostly related to oxidative stress, DNA damage and autophagy. This is the deepest characterization of the boar sperm transcriptome and the first study linking the transcriptome and the seasonal variability of semen quality in animals. The annotation described here can be used as a reference for the identification of markers of sperm quality in pigs.
Grants:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1528 Ministerio de Economía y Competitividad AGL2013-44978-R Ministerio de Economía y Competitividad AGL2017-86946-R Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad BES-2014-070560 Ministerio de Economía y Competitividad EEBB-I-2017-12229 Ministerio de Economía y Competitividad RYC-2011-07763
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Frontiers in genetics, Vol. 10 (April 2019) , art. 299, ISSN 1664-8021

DOI: 10.3389/fgene.2019.00299
PMID: 31040860

14 p, 1.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles